Latest advances in cryogel technology for biomedical applications

A Memic, T Colombani, LJ Eggermont… - Advanced …, 2019 - Wiley Online Library
There exists a technological need for advanced materials with improved properties for
emerging biomedical applications. Recent developments in macroporous materials have …

Lentiviral vector bioprocessing

C Perry, ACME Rayat - Viruses, 2021 - mdpi.com
Lentiviral vectors (LVs) are potent tools for the delivery of genes of interest into mammalian
cells and are now commonly utilised within the growing field of cell and gene therapy for the …

Production of lentiviral vectors

OW Merten, M Hebben, C Bovolenta - Molecular Therapy-Methods & …, 2016 - cell.com
Lentiviral vectors (LV) have seen considerably increase in use as gene therapy vectors for
the treatment of acquired and inherited diseases. This review presents the state of the art of …

Current challenges in biotherapeutic particles manufacturing

MG Moleirinho, RJS Silva, PM Alves… - Expert opinion on …, 2020 - Taylor & Francis
Introduction: The development of novel complex biotherapeutics led to new challenges in
biopharmaceutical industry. The potential of these particles has been demonstrated by the …

Large-scale production of lentiviral vectors: current perspectives and challenges

E Martínez-Molina, C Chocarro-Wrona… - Pharmaceutics, 2020 - mdpi.com
Lentiviral vectors (LVs) have gained value over recent years as gene carriers in gene
therapy. These viral vectors are safer than what was previously being used for gene transfer …

New developments in lentiviral vector design, production and purification

MM Segura, M Mangion, B Gaillet… - Expert opinion on …, 2013 - Taylor & Francis
Introduction: Lentiviruses are a very potent class of viral vectors for which there is presently a
rapidly growing interest for a number of gene therapy. However, their construction …

Polymethacrylate monoliths for preparative and industrial separation of biomolecular assemblies

A Jungbauer, R Hahn - Journal of chromatography A, 2008 - Elsevier
Monoliths are considered as the fourth-generation chromatography material. Their use for
preparative separation of biomolecules has been evolved over the past decade. Monolithic …

Advances in lentivirus purification

AS Moreira, DG Cavaco, TQ Faria… - Biotechnology …, 2021 - Wiley Online Library
Lentiviral vectors (LVs) have been increasingly used as a tool for gene and cell therapies
since they can stably integrate the genome in dividing and nondividing cells. LV production …

Improved virus purification processes for vaccines and gene therapy

P Nestola, C Peixoto, RRJS Silva… - Biotechnology and …, 2015 - Wiley Online Library
The downstream processing of virus particles for vaccination or gene therapy is becoming a
critical bottleneck as upstream titers keep improving. Moreover, the growing pressure to …

Challenges of up-scaling lentivirus production and processing

A McCarron, M Donnelley, C McIntyre… - Journal of biotechnology, 2016 - Elsevier
Lentiviruses are becoming an increasingly popular choice of gene transfer vehicle for use in
the treatment of a variety of genetic and acquired human diseases. As research progresses …