Recent advances in various 'omics' technologies enable quantitative monitoring of the
abundance of various biological molecules in a high-throughput manner, and thus allow …

Software-based image analysis is a crucial step in the biological interpretation of two-
dimensional gel electrophoresis experiments. Recent significant advances in image …

iTRAQ (isobaric tags for relative or absolute quantitation) is a mass spectrometry technology
that allows quantitative comparison of protein abundance by measuring peak intensities of …

The proteome refers to the collection of proteins in a given biological organism or system
under a particular set of environmental conditions. The study of proteins, referred to as …

Given the rapid developments in mass spectrometry (MS) in terms of sensitivity, mass
accuracy, and throughput, some have suggested that two-dimensional gel electrophoresis …

Chitosan, a partially deacetylated form of the natural and biodegradable biopolymer chitin,
has been used as plant growth promoter in agriculture. The aim of this work was to …

This work presents a comparative evaluation of several detergent‐based sample
preparation workflows for the MS‐based analysis of bacterial proteomes, performed using …

Bradyrhizobium japonicum, a gram‐negative soil bacterium that establishes an N2‐fixing
symbiosis with its legume host soybean (Glycine max), has been used as a symbiosis model …

Abstract Background Sigma factor B (SigB) is the central regulator of the general stress
response in Bacillus subtilis and regulates a group of genes in response to various …

We applied stable isotope labeling by amino acids in cell culture (SILAC) to large-scale
quantitative proteomics analyses of the model bacterium Bacillus subtilis in two …