New and rapid visual detection assay for Trogoderma granarium everts based on recombinase polymerase amplification and CRISPR/Cas12a

L Zeng, S Zheng, V Stejskal, G Opit… - Pest Management …, 2023 - Wiley Online Library
BACKGROUND Khapra beetle (Trogoderma granarium Everts), one of the most important
quarantine pests globally, is capable of causing severe infestation and huge economic loss …

Application of recombinase polymerase amplification with CRISPR/Cas12a and multienzyme isothermal rapid amplification with lateral flow dipstick assay for …

W Li, B Cai, R Chen, J Cui, H Wang… - Pest Management …, 2024 - Wiley Online Library
BACKGROUND Bactrocera correcta is a quarantine pest that negatively impacts the fruit and
vegetable industry. Differentiating B. correcta from similar species, especially in non‐adult …

Development of a CRISPR/Cas12a-facilitated fluorescent aptasensor for sensitive detection of small molecules

L Li, M Li, S Wang, Y Dong - International Journal of Biological …, 2024 - Elsevier
Abstract The integration of Clustered Regularly Interspaced Short Palindromic Repeats
(CRISPR) and CRISPR-associated proteins (Cas) exhibits superior performance in …

Rapid and Sensitive On-site Nucleic Acid Detection of Three Main Fusarium Pathogens of Maize Stalk Rot Based on RPA-CRISPR/Cas12a

F Jiang, X Ding, X Wang, K Fu, Z Jia, L Liang… - Plant …, 2024 - Am Phytopath Society
Maize stalk rot is a soil-borne disease that poses a serious threat to maize production
worldwide, with the most significant cause being fungal stalk rot. The development of a …

Rapid and equipment‐free identification of papaya mealybug Paracoccus marginatus based on RPA‐CRISPR/Cas12a

YT Chen, MZ Shi, Y Chen, JW Zhao, XJ Yang… - Pest Management … - Wiley Online Library
BACKGROUND Paracoccus marginatus has invaded many countries, spreading rapidly and
causing significant economic losses to crops. Accurate detection during the monitoring …

[PDF][PDF] New and rapid visual detection assay for

L Zeng, S Zheng, V Stejskal, G Opit, R Aulickyd, Z Lia - 2023 - researchgate.net
BACKGROUND: Khapra beetle (Trogoderma granarium Everts), one of the most important
quarantine pests globally, is capable of causing severe infestation and huge economic loss …