In trypanosomes, RNA polymerase II transcription is polycistronic and individual mRNAs are
excised by trans-splicing and polyadenylation. The lack of individual gene transcription …

DNA methylation plays an important role in the maintenance of genomic stability. Ten-
eleven translocation proteins (TETs) are a family of iron (Fe2+) and α-KG-dependent …

Mapping open chromatin regions has emerged as a widely used tool for identifying active
regulatory elements in eukaryotes. However, existing approaches, limited by reliance on …

The protozoan Trypanosoma cruzi almost invariably establishes life-long infections in
humans and other mammals, despite the development of potent host immune responses …

Conspectus Protein–DNA interactions are important in replication, transcription, repair, as
well as epigenetic modifications of DNA, which involve methylation and demethylation of …

Chemically modified bases exist naturally in genomic DNA. Research into these bases has
been invigorated by the discovery of several modified bases in the mammalian genome, in …

Multicellular organisms developed the concept of specialized cells that perform specific
functions. Examples are neurons and fibroblast to name just two out of more than 200 …

While some DNA base modifications such as 5-methylcytosine have been known and
studied for decades, recent discoveries of a number of other modified bases have stimulated …

In DNA, the loss of a nucleobase by hydrolysis generates an abasic site. Formed as a result
of DNA damage, as well as a key intermediate during the base excision repair pathway …

Leishmaniasis is one of the major neglected tropical diseases, for which no vaccines exist.
Chemotherapy is hampered by limited efficacy coupled with development of resistance and …