Light sheet fluorescence microscopy

EHK Stelzer, F Strobl, BJ Chang, F Preusser… - Nature Reviews …, 2021 - nature.com
Light sheet fluorescence microscopy (LSFM) uses a thin sheet of light to excite only
fluorophores within the focal volume. Light sheet microscopes (LSMs) have a true optical …

Current progress in expansion microscopy: chemical strategies and applications

G Wen, V Leen, T Rohand, M Sauer… - Chemical …, 2023 - ACS Publications
Expansion microscopy (ExM) is a newly developed super-resolution technique, allowing
visualization of biological targets at nanoscale resolution on conventional fluorescence …

SARS-CoV-2 nucleocapsid protein adheres to replication organelles before viral assembly at the Golgi/ERGIC and lysosome-mediated egress

KM Scherer, L Mascheroni, GW Carnell… - Science …, 2022 - science.org
Despite being the target of extensive research efforts due to the COVID-19 (coronavirus
disease 2019) pandemic, relatively little is known about the dynamics of severe acute …

Expansion microscopy: A chemical approach for super-resolution microscopy

Y Zhuang, X Shi - Current opinion in structural biology, 2023 - Elsevier
Super-resolution microscopy is a series of imaging techniques that bypass the diffraction
limit of resolution. Since the 1990s, optical approaches, such as single-molecular …

Multiscale imaging informs translational mouse modeling of neurological disease

Y Wang, JM LeDue, TH Murphy - Neuron, 2022 - cell.com
Multiscale neurophysiology reveals that simple motor actions are associated with changes
in neuronal firing in virtually every brain region studied. Accordingly, the assessment of focal …

Subdiffraction-resolution fluorescence imaging of immunological synapse formation between NK cells and A. fumigatus by expansion microscopy

N Trinks, S Reinhard, M Drobny, L Heilig… - Communications …, 2021 - nature.com
Expansion microscopy (ExM) enables super-resolution fluorescence imaging on standard
microscopes by physical expansion of the sample. However, the investigation of interactions …

Towards a quantitative single particle characterization by super resolution microscopy: From virus structures to antivirals design

M Arista-Romero, S Pujals, L Albertazzi - Frontiers in Bioengineering …, 2021 - frontiersin.org
In the last year the COVID19 pandemic clearly illustrated the potential threat that viruses
pose to our society. The characterization of viral structures and the identification of key …

Application of super-resolution and advanced quantitative microscopy to the spatio-temporal analysis of influenza virus replication

E Touizer, C Sieben, R Henriques, M Marsh, RF Laine - Viruses, 2021 - mdpi.com
With an estimated three to five million human cases annually and the potential to infect
domestic and wild animal populations, influenza viruses are one of the greatest health and …

Expanding boundaries–a cell biologist's guide to expansion microscopy

N Hümpfer, R Thielhorn, H Ewers - Journal of Cell Science, 2024 - journals.biologists.com
Expansion microscopy (ExM) is a revolutionary novel approach to increase resolution in
light microscopy. In contrast to super-resolution microscopy methods that rely on …

Expansion and Light‐Sheet Microscopy for Nanoscale 3D Imaging

L Pesce, P Ricci, G Sportelli, N Belcari… - Small …, 2024 - Wiley Online Library
Abstract Expansion Microscopy (ExM) and Light‐Sheet Fluorescence Microscopy (LSFM)
are forefront imaging techniques that enable high‐resolution visualization of biological …