Cell-free gene expression

D Garenne, MC Haines, EF Romantseva… - Nature reviews …, 2021 - nature.com
Cell-free gene expression (CFE) emerged as an alternative approach to living cells for
specific applications in protein synthesis and labelling for structural biology and proteomics …

Cell-free gene expression: methods and applications

AC Hunt, BJ Rasor, K Seki, HM Ekas, KF Warfel… - Chemical …, 2024 - ACS Publications
Cell-free gene expression (CFE) systems empower synthetic biologists to build biological
molecules and processes outside of living intact cells. The foundational principle is that …

Advances in cell‐free biosensors: principle, mechanism, and applications

L Zhang, W Guo, Y Lu - Biotechnology Journal, 2020 - Wiley Online Library
Synthetic biology has promoted the development of biosensors as tools for detecting trace
substances. In the past, biosensors based on synthetic biology have been designed on …

Microbial biomanufacturing for space-exploration—what to take and when to make

NJH Averesch, AJ Berliner, SN Nangle… - Nature …, 2023 - nature.com
As renewed interest in human space-exploration intensifies, a coherent and modernized
strategy for mission design and planning has become increasingly crucial. Biotechnology …

[HTML][HTML] Synthetic biology as driver for the biologization of materials sciences

O Burgos-Morales, M Gueye, L Lacombe, C Nowak… - Materials Today Bio, 2021 - Elsevier
Materials in nature have fascinating properties that serve as a continuous source of
inspiration for materials scientists. Accordingly, bio-mimetic and bio-inspired approaches …

Rational design of functional amyloid fibrillar assemblies

X Wang, S Zhang, J Zhang, Y Wang, X Jiang… - Chemical Society …, 2023 - pubs.rsc.org
Amyloid fibrillar assemblies, originally identified as pathological entities in
neurodegenerative diseases, have been widely adopted by various proteins to fulfill diverse …

Cell-free biocatalysis for the production of platform chemicals

PL Bergquist, S Siddiqui, A Sunna - Frontiers in Energy Research, 2020 - frontiersin.org
Genetically engineered host bacteria have an extensive history for the production of specific
proteins including the synthesis of single enzymes for the modification of compounds …

Taxonomy for engineered living materials

AD Lantada, JG Korvink, M Islam - Cell Reports Physical Science, 2022 - cell.com
Engineered living materials (ELMs) are the most relevant contemporary revolution in
materials science and engineering and aim to outperform current examples of" smart," …

Harnessing the central dogma for stringent multi-level control of gene expression

FV Greco, A Pandi, TJ Erb, CS Grierson… - Nature …, 2021 - nature.com
Strictly controlled inducible gene expression is crucial when engineering biological systems
where even tiny amounts of a protein have a large impact on function or host cell viability. In …

Cell-free protein synthesis system for bioanalysis: Advances in methods and applications

Y Gu, F Fan, Y Liu, Y Chai, Y Yuan, X Chen - TrAC Trends in Analytical …, 2023 - Elsevier
Efficient, dynamic and accurate are the iterative trends of modern bioanalysis. Current cell-
based platforms are hindered by time-consuming cell culture cycles, physiological …