Digital Recombinase Polymerase Amplification, Digital Loop‐Mediated Isothermal Amplification, and Digital CRISPR‐Cas Assisted Assay: Current Status, Challenges …
W Yin, J Zhuang, J Li, L Xia, K Hu, J Yin, Y Mu - Small, 2023 - Wiley Online Library
Digital nucleic acid detection based on microfluidics technology can quantify the initial
amount of nucleic acid in the sample with low equipment requirements and simple …
amount of nucleic acid in the sample with low equipment requirements and simple …
CRISPR for companion diagnostics in low-resource settings
Q Xu, Q Xu, C Lyon, TY Hu - Lab on a Chip, 2024 - pubs.rsc.org
New point-of-care tests (POCTs), which are especially useful in low-resource settings, are
needed to expand screening capacity for diseases that cause significant mortality …
needed to expand screening capacity for diseases that cause significant mortality …
[HTML][HTML] Quantitative detection of circular RNA and microRNA at point-of-care using droplet digital CRISPR/Cas13a platform
K Wang, H Yin, S Li, Y Wan, M Xiao, X Yuan… - Biosensors and …, 2025 - Elsevier
Circular RNA (circRNA) and microRNA (miRNA) are both non-coding RNAs (ncRNAs) that
serve as biomarkers for cancer diagnosis and prognosis. Quantitative detection of these …
serve as biomarkers for cancer diagnosis and prognosis. Quantitative detection of these …
Empowering the on-site detection of nucleic acids by integrating CRISPR and digital signal processing
Addressing the global disparity in cancer care necessitates the development of rapid and
affordable nucleic acid (NA) testing technologies. This need is particularly critical for cervical …
affordable nucleic acid (NA) testing technologies. This need is particularly critical for cervical …
CRISPR-powered strategies for amplification-free diagnostics of infectious diseases
Y Xia, R Rao, M Xiong, B He, B Zheng, Y Jia… - Analytical …, 2024 - ACS Publications
Infectious diseases continue to present significant challenges globally, with high mortality
rates and escalating healthcare costs. The Clustered Regularly Interspaced Short …
rates and escalating healthcare costs. The Clustered Regularly Interspaced Short …
DECODE: Contamination-Free Digital CRISPR Platform for Point-of-Care Detection of Viral DNA/RNA
S Li, H Yin, J Zheng, Y Wan, K Wang, C Yang… - ACS …, 2024 - ACS Publications
Rapid and precise nucleic acid testing at the point-of-care (POC) is essential for effective
screening and management of infectious diseases. Current polymerase-based molecular …
screening and management of infectious diseases. Current polymerase-based molecular …
[HTML][HTML] Harnessing CRISPR/Cas Systems for DNA and RNA Detection: Principles, Techniques, and Challenges
H Son - Biosensors, 2024 - mdpi.com
The emergence of CRISPR/Cas systems has revolutionized the field of molecular
diagnostics with their high specificity and sensitivity. This review provides a comprehensive …
diagnostics with their high specificity and sensitivity. This review provides a comprehensive …
Single microentity analysis-based ultrasensitive bioassays: Recent advances, applications, and perspectives
W Fan, Y Dong, W Ren, C Liu - TrAC Trends in Analytical Chemistry, 2023 - Elsevier
The development of ultrasensitive biomarker analysis strategies is of great significance in
early disease diagnosis and human health. Superior to the conventional homogenous bulk …
early disease diagnosis and human health. Superior to the conventional homogenous bulk …
Machine Learning-Assisted, Dual-Channel CRISPR/Cas12a Biosensor-In-Microdroplet for Amplification-Free Nucleic Acid Detection for Food Authenticity Testing
Z Zhao, R Wang, X Yang, J Jia, Q Zhang, S Ye, S Man… - ACS …, 2024 - ACS Publications
The development of novel detection technology for meat species authenticity is imperative.
Here, we developed a machine learning-supported, dual-channel biosensor-in-microdroplet …
Here, we developed a machine learning-supported, dual-channel biosensor-in-microdroplet …
Advancements in CRISPR-Diagnostic Techniques for Rapid on-site Monitoring of Environmental Virus
Z Deng, W Liao, L Bu, J Li, J Li, L Wang, A Yuan… - TrAC Trends in …, 2024 - Elsevier
Highly sensitive and rapid environmental surveillance is crucial due to the significant role of
various environmental media in transmitting multiple viruses. Specifically, when examining …
various environmental media in transmitting multiple viruses. Specifically, when examining …