Digital Recombinase Polymerase Amplification, Digital Loop‐Mediated Isothermal Amplification, and Digital CRISPR‐Cas Assisted Assay: Current Status, Challenges …

W Yin, J Zhuang, J Li, L Xia, K Hu, J Yin, Y Mu - Small, 2023 - Wiley Online Library
Digital nucleic acid detection based on microfluidics technology can quantify the initial
amount of nucleic acid in the sample with low equipment requirements and simple …

CRISPR for companion diagnostics in low-resource settings

Q Xu, Q Xu, C Lyon, TY Hu - Lab on a Chip, 2024 - pubs.rsc.org
New point-of-care tests (POCTs), which are especially useful in low-resource settings, are
needed to expand screening capacity for diseases that cause significant mortality …

[HTML][HTML] Quantitative detection of circular RNA and microRNA at point-of-care using droplet digital CRISPR/Cas13a platform

K Wang, H Yin, S Li, Y Wan, M Xiao, X Yuan… - Biosensors and …, 2025 - Elsevier
Circular RNA (circRNA) and microRNA (miRNA) are both non-coding RNAs (ncRNAs) that
serve as biomarkers for cancer diagnosis and prognosis. Quantitative detection of these …

Empowering the on-site detection of nucleic acids by integrating CRISPR and digital signal processing

CY Lee, H Kim, I Degani, H Lee, A Sandoval… - Nature …, 2024 - nature.com
Addressing the global disparity in cancer care necessitates the development of rapid and
affordable nucleic acid (NA) testing technologies. This need is particularly critical for cervical …

CRISPR-powered strategies for amplification-free diagnostics of infectious diseases

Y Xia, R Rao, M Xiong, B He, B Zheng, Y Jia… - Analytical …, 2024 - ACS Publications
Infectious diseases continue to present significant challenges globally, with high mortality
rates and escalating healthcare costs. The Clustered Regularly Interspaced Short …

DECODE: Contamination-Free Digital CRISPR Platform for Point-of-Care Detection of Viral DNA/RNA

S Li, H Yin, J Zheng, Y Wan, K Wang, C Yang… - ACS …, 2024 - ACS Publications
Rapid and precise nucleic acid testing at the point-of-care (POC) is essential for effective
screening and management of infectious diseases. Current polymerase-based molecular …

[HTML][HTML] Harnessing CRISPR/Cas Systems for DNA and RNA Detection: Principles, Techniques, and Challenges

H Son - Biosensors, 2024 - mdpi.com
The emergence of CRISPR/Cas systems has revolutionized the field of molecular
diagnostics with their high specificity and sensitivity. This review provides a comprehensive …

Single microentity analysis-based ultrasensitive bioassays: Recent advances, applications, and perspectives

W Fan, Y Dong, W Ren, C Liu - TrAC Trends in Analytical Chemistry, 2023 - Elsevier
The development of ultrasensitive biomarker analysis strategies is of great significance in
early disease diagnosis and human health. Superior to the conventional homogenous bulk …

Machine Learning-Assisted, Dual-Channel CRISPR/Cas12a Biosensor-In-Microdroplet for Amplification-Free Nucleic Acid Detection for Food Authenticity Testing

Z Zhao, R Wang, X Yang, J Jia, Q Zhang, S Ye, S Man… - ACS …, 2024 - ACS Publications
The development of novel detection technology for meat species authenticity is imperative.
Here, we developed a machine learning-supported, dual-channel biosensor-in-microdroplet …

Advancements in CRISPR-Diagnostic Techniques for Rapid on-site Monitoring of Environmental Virus

Z Deng, W Liao, L Bu, J Li, J Li, L Wang, A Yuan… - TrAC Trends in …, 2024 - Elsevier
Highly sensitive and rapid environmental surveillance is crucial due to the significant role of
various environmental media in transmitting multiple viruses. Specifically, when examining …