Technologies enabling single-molecule super-resolution imaging of mRNA

M Tingey, SJ Schnell, W Yu, J Saredy, S Junod, D Patel… - Cells, 2022 - mdpi.com
The transient nature of RNA has rendered it one of the more difficult biological targets for
imaging. This difficulty stems both from the physical properties of RNA as well as the …

Spelling out the roles of individual nucleoporins in nuclear export of mRNA

M Tingey, Y Li, W Yu, A Young, W Yang - Nucleus, 2022 - Taylor & Francis
ABSTRACT The Nuclear Pore Complex (NPC) represents a critical passage through the
nuclear envelope for nuclear import and export that impacts nearly every cellular process at …

High-speed super-resolution imaging of rotationally symmetric structures using SPEED microscopy and 2D-to-3D transformation

Y Li, M Tingey, A Ruba, W Yang - Nature protocols, 2021 - nature.com
Various super-resolution imaging techniques have been developed to break the diffraction-
limited resolution of light microscopy. However, it still remains challenging to obtain three …

The ciliary lumen accommodates passive diffusion and vesicle-assisted trafficking in cytoplasm–ciliary transport

A Ruba, M Tingey, W Luo, J Yu… - Molecular Biology of …, 2023 - Am Soc Cell Biol
Transport of membrane and cytosolic proteins into the primary cilium is essential for its role
in cellular signaling. Using virtual three-dimensional superresolution light microscopy, the …

Unraveling docking and initiation of mRNA export through the nuclear pore complex

M Tingey, W Yang - Bioessays, 2022 - Wiley Online Library
The nuclear export of mRNA through the nuclear pore complex (NPC) is a process required
for the healthy functioning of human cells, making it a critical area of research. However, the …

[HTML][HTML] Obtaining 3D super-resolution images by utilizing rotationally symmetric structures and 2D-to-3D transformation

SL Junod, M Tingey, C Rush, A Alkurdi… - Computational and …, 2023 - Elsevier
Super-resolution imaging techniques have provided unprecedentedly detailed information
by surpassing the diffraction-limited resolution of light microscopy. However, in order to …

Nucleocytoplasmic transport of intrinsically disordered proteins studied by high‐speed super‐resolution microscopy

SL Junod, JM Kelich, J Ma, W Yang - Protein Science, 2020 - Wiley Online Library
Both natively folded and intrinsically disordered proteins (IDPs) destined for the nucleus
need to transport through the nuclear pore complexes (NPCs) in eukaryotic cells. NPCs …

Application of Super-resolution SPEED Microscopy in the Study of Cellular Dynamics

W Yu, C Rush, M Tingey, S Junod… - Chemical & Biomedical …, 2023 - ACS Publications
Super-resolution imaging techniques have broken the diffraction-limited resolution of light
microscopy. However, acquiring three-dimensional (3D) super-resolution information about …

Speed microscopy: high-speed single molecule tracking and mapping of nucleocytoplasmic transport

SJ Schnell, M Tingey, W Yang - The Nuclear Pore Complex: Methods and …, 2022 - Springer
The nuclear pore complex (NPC) functions as a gateway through which molecules
translocate into and out of the nucleus. Understanding the transport dynamics of these …

Deciphering vesicle-assisted transport mechanisms in cytoplasm to cilium trafficking

M Tingey, A Ruba, Z Jiang, W Yang - Frontiers in Cellular …, 2024 - frontiersin.org
The cilium, a pivotal organelle crucial for cell signaling and proper cell function, relies on
meticulous macromolecular transport from the cytoplasm for its formation and maintenance …