[HTML][HTML] Emergent CRISPR–Cas-based technologies for engineering non-model bacteria
Highlights•CRISPR–Cas systems promoted the engineering of non-model bacteria.•Tools
include genome modifications and finely-tuned transcriptional regulation.•CRISPR–Cas …
include genome modifications and finely-tuned transcriptional regulation.•CRISPR–Cas …
[HTML][HTML] Advances in systems metabolic engineering of autotrophic carbon oxide-fixing biocatalysts towards a circular economy
High levels of anthropogenic CO 2 emissions are driving the warming of global climate. If
this pattern of increasing emissions does not change, it will cause further climate change …
this pattern of increasing emissions does not change, it will cause further climate change …
Towards continuous industrial bioprocessing with solventogenic and acetogenic clostridia: challenges, progress and perspectives
CA Vees, CS Neuendorf, S Pflügl - Journal of Industrial …, 2020 - academic.oup.com
The sustainable production of solvents from above ground carbon is highly desired. Several
clostridia naturally produce solvents and use a variety of renewable and waste-derived …
clostridia naturally produce solvents and use a variety of renewable and waste-derived …
Genetic and metabolic engineering challenges of C1-gas fermenting acetogenic chassis organisms
Unabated mining and utilisation of petroleum and petroleum resources and their conversion
to essential fuels and chemicals have drastic environmental consequences, contributing to …
to essential fuels and chemicals have drastic environmental consequences, contributing to …
[HTML][HTML] Synthetic biology on acetogenic bacteria for highly efficient conversion of C1 gases to biochemicals
Synthesis gas, which is mainly produced from fossil fuels or biomass gasification, consists of
C1 gases such as carbon monoxide, carbon dioxide, and methane as well as hydrogen …
C1 gases such as carbon monoxide, carbon dioxide, and methane as well as hydrogen …
Developing a Base Editing System for Marine Roseobacter Clade Bacteria
The Roseobacter clade bacteria are of great significance in marine ecology and
biogeochemical cycles, and they are potential microbial chassis for marine synthetic biology …
biogeochemical cycles, and they are potential microbial chassis for marine synthetic biology …
[HTML][HTML] Engineering acetogenic bacteria for efficient one-carbon utilization
C1 gases, including carbon dioxide (CO2) and carbon monoxide (CO), are major
contributors to climate crisis. Numerous studies have been conducted to fix and recycle C1 …
contributors to climate crisis. Numerous studies have been conducted to fix and recycle C1 …
[HTML][HTML] Orthogonal LoxPsym sites allow multiplexed site-specific recombination in prokaryotic and eukaryotic hosts
C Cautereels, J Smets, J De Saeger, L Cool… - Nature …, 2024 - nature.com
Site-specific recombinases such as the Cre-LoxP system are routinely used for genome
engineering in both prokaryotes and eukaryotes. Importantly, recombinases complement the …
engineering in both prokaryotes and eukaryotes. Importantly, recombinases complement the …
Base editing for reprogramming cyanobacterium Synechococcus elongatus
SY Wang, X Li, SG Wang, PF Xia - Metabolic Engineering, 2023 - Elsevier
Cyanobacteria can directly convert carbon dioxide (CO 2) at the atmospheric level to
biofuels, value-added chemicals and food products, making them ideal candidates to …
biofuels, value-added chemicals and food products, making them ideal candidates to …
Development of a base editor for protein evolution via in situ mutation in vivo
W Hao, W Cui, Z Cheng, L Han, F Suo… - Nucleic Acids …, 2021 - academic.oup.com
Protein evolution has significantly enhanced the development of life science. However, it is
difficult to achieve in vitro evolution of some special proteins because of difficulties with …
difficult to achieve in vitro evolution of some special proteins because of difficulties with …