Highlights•CRISPR–Cas systems promoted the engineering of non-model bacteria.•Tools
include genome modifications and finely-tuned transcriptional regulation.•CRISPR–Cas …

High levels of anthropogenic CO 2 emissions are driving the warming of global climate. If
this pattern of increasing emissions does not change, it will cause further climate change …

The sustainable production of solvents from above ground carbon is highly desired. Several
clostridia naturally produce solvents and use a variety of renewable and waste-derived …

Unabated mining and utilisation of petroleum and petroleum resources and their conversion
to essential fuels and chemicals have drastic environmental consequences, contributing to …

Synthesis gas, which is mainly produced from fossil fuels or biomass gasification, consists of
C1 gases such as carbon monoxide, carbon dioxide, and methane as well as hydrogen …

The Roseobacter clade bacteria are of great significance in marine ecology and
biogeochemical cycles, and they are potential microbial chassis for marine synthetic biology …

C1 gases, including carbon dioxide (CO2) and carbon monoxide (CO), are major
contributors to climate crisis. Numerous studies have been conducted to fix and recycle C1 …

Site-specific recombinases such as the Cre-LoxP system are routinely used for genome
engineering in both prokaryotes and eukaryotes. Importantly, recombinases complement the …

Cyanobacteria can directly convert carbon dioxide (CO 2) at the atmospheric level to
biofuels, value-added chemicals and food products, making them ideal candidates to …

Protein evolution has significantly enhanced the development of life science. However, it is
difficult to achieve in vitro evolution of some special proteins because of difficulties with …