Riboswitches are common gene regulatory units mostly found in bacteria that are capable of
altering gene expression in response to a small molecule. These structured RNA elements …

In synthetic biology, metabolic engineering, and gene therapy, there is a strong demand for
orthogonal or externally controlled regulation of gene expression. Here, RNA-based …

Widespread use of gene therapy technologies is limited in part by the lack of small genetic
switches with wide dynamic ranges that control transgene expression without the …

RNA utilizes many different mechanisms to control gene expression. Among the regulatory
elements that respond to external stimuli, riboswitches are a prominent and elegant …

Nature has evolved a treasury of biological molecules that are logically connected to
networks, enabling cells to maintain their functional integrity. Similar to electronic circuits …

Efforts to control mammalian gene expression with ligand-responsive riboswitches have
been hindered by lack of a general method for generating efficient switches in mammalian …

Highlights•Addition of in vivo active aptamer domains for DNT and PPDA to the
toolbox.•Addition of expression platforms for transcriptional termination to the …

Robust synthetic devices are requisite for the construction of synthetic genetic circuits and
important scientific and technological tools to control cellular processes. We developed …

Aptazymes are small, ligand-dependent self-cleaving ribozymes that function independently
of transcription factors and can be customized for induction by various small molecules …

The past decade of synthetic biology research has witnessed numerous advances in the
development of tools and frameworks for the design and characterization of biological …