The amino acid sequence of a protein affects both its structure and its function. Thus, the
ability to modify the sequence, and hence the structure and activity, of individual proteins in …

Directed evolution is a common technique to engineer enzymes for a diverse set of
applications. Structural information and an understanding of how proteins respond to …

Alcohol-forming fatty acyl reductases (FARs) catalyze the reduction of thioesters to alcohols
and are key enzymes for microbial production of fatty alcohols. Many metabolic engineering …

SCHEMA structure-guided recombination of 3 fungal class II cellobiohydrolases (CBH II
cellulases) has yielded a collection of highly thermostable CBH II chimeras. Twenty-three of …

Systematic approaches to directed evolution of proteins have been documented since the
1970s. The ability to recruit new protein functions arises from the considerable substrate …

Directed evolution continues to expand the capabilities of complex biomolecules for a range
of applications, such as adeno-associated virus vectors for gene therapy; however …

Protein engineering has nearly limitless applications across chemistry, energy and
medicine, but creating new proteins with improved or novel functions remains slow, labor …

Today, adeno-associated virus (AAV) is an extremely popular choice for gene therapy
delivery. The safety profile and simplicity of the genome organization are the decisive …

Creating artificial protein families affords new opportunities to explore the determinants of
structure and biological function free from many of the constraints of natural selection. We …

Generative probabilistic models of biological sequences have widespread existing and
potential applications in analyzing, predicting and designing proteins, RNA and genomes …