The emerging inverse electron demand Diels–Alder (IEDDA) reaction stands out from other
bioorthogonal reactions by virtue of its unmatchable kinetics, excellent orthogonality and …

Bioorthogonal reactions have found widespread use in applications ranging from glycan
engineering to in vivo imaging. Researchers have devised numerous reactions that can be …

Over 500 natural and synthetic amino acids have been genetically encoded in the last two
decades. Incorporating these noncanonical amino acids into proteins enables many …

Over the past 16 years, genetic code expansion and reprogramming in living organisms has
been transformed by advances that leverage the unique properties of pyrrolysyl-tRNA …

Reactions that occur under physiological conditions find diverse uses in the chemical and
biological sciences. However, the limitations that biological systems place on chemical …

Localized surface plasmon resonance (LSPR)-based biosensors have recently garnered
increasing attention due to their potential to allow label-free, portable, low-cost, and real …

With few-nanometer resolution recently achieved by a new generation of fluorescence
nanoscopes (MINFLUX and MINSTED), the size of the tags used to label proteins will …

Modern light microscopy, including super-resolution techniques, has brought about a
demand for small labeling tags that bring the fluorophore closer to the target. This challenge …

Genetic code expansion (GCE) technology allows the specific incorporation of
functionalized noncanonical amino acids (ncAAs) into proteins. Here, we investigated the …

Super‐resolution microscopy (SRM) greatly benefits from the ability to install small
photostable fluorescent labels into proteins. Genetic code expansion (GCE) technology …