Traditional biochemical methods for enzyme detection are mainly based on antibody-based
immunoassays, which lack the ability to monitor the spatiotemporal distribution and, in …

The sensitivity of fluorescence polarization (FP) and fluorescence anisotropy (FA) to
molecular weight changes has enabled the interrogation of diverse biological mechanisms …

Introduction: Fluorescence anisotropy (FA) is one of the major established methods
accepted by industry and regulatory agencies for understanding the mechanisms of drug …

Apical caspases initiate and effector caspases execute apoptosis. Reagents that can
distinguish between caspases, particularly apical caspases-8, 9, and 10 are scarce and …

PAC-1 induces the activation of procaspase-3 in vitro and in cell culture by chelation of
inhibitory labile zinc ions via its ortho-hydroxy-N-acylhydrazone moiety. First reported in …

Caspases, aspartate-specific cysteine proteases, have fate-determining roles in many
cellular processes including apoptosis, differentiation, neuronal remodeling, and …

The antitumor activity of natural flavonoids has been exhaustively reported. Previously it has
been demonstrated that prenylation of flavonoids allows the discovery of new compounds …

Caspases are a family of cysteine proteases that are well-known for their roles in apoptosis
and inflammation. Recent studies provide evidence that caspases are also integral to many …

Procaspase-activating compound 1 (PAC-1) is an o-hydroxy-N-acylhydrazone that induces
apoptosis in cancer cells by chelation of labile inhibitory zinc from procaspase-3. PAC-1 has …

Lysozyme is a well-known enzyme found in many biological fluids which plays an important
role in the antibacterial protection of humans and animals. Lysozyme assays are used for …