Illuminating cellular architecture and dynamics with fluorescence polarization microscopy

WF Dean, AL Mattheyses - Journal of Cell Science, 2024 - journals.biologists.com
ABSTRACT Ever since Robert Hooke's 17th century discovery of the cell using a humble
compound microscope, light–matter interactions have continuously redefined our …

[HTML][HTML] High affinity heme binding to a heme regulatory motif on the nuclear receptor Rev-erbβ leads to its degradation and indirectly regulates its interaction with …

EL Carter, N Gupta, SW Ragsdale - Journal of Biological Chemistry, 2016 - ASBMB
Rev-erbα and Rev-erbβ are heme-binding nuclear receptors (NR) that repress the
transcription of genes involved in regulating metabolism, inflammation, and the circadian …

[HTML][HTML] Organization of cytoplasmic domains of sarcoplasmic reticulum Ca2+-ATPase in E1P and E1ATP states: a limited proteolysis study

S Danko, K Yamasaki, T Daiho, H Suzuki, C Toyoshima - FEBS letters, 2001 - Elsevier
In order to characterize the domain organization of sarcoplasmic reticulum Ca2+-ATPase in
different physiological states, limited proteolysis using three proteases (proteinase K (prtK) …

[HTML][HTML] Deletions of any single residues in Glu40-Ser48 loop connecting a domain and the first transmembrane helix of sarcoplasmic reticulum Ca2+-ATPase result in …

T Daiho, K Yamasaki, G Wang, S Danko… - Journal of Biological …, 2003 - ASBMB
Possible roles of the Glu 40-Ser 48 loop connecting A domain and the first transmembrane
helix (M1) in sarcoplasmic reticulum Ca 2+-ATPase (SERCA1a) were explored by …

Effects of Ranitidine on Gastric Vesicles Containing H+, K+-Adenosine Triphosphatase in Rats

K Kubo, A Uehara, T Kubota, T Nozu… - Scandinavian journal …, 1995 - Taylor & Francis
Background: To ascertain the mechanism for rebound acid hypersecretion after treatment
with an H2-receptor blocker, we investigated the effects of ranitidine on gastric H+, K+ …

SERCA structural dynamics induced by ATP and calcium

B Mueller, M Zhao, IV Negrashov, R Bennett… - Biochemistry, 2004 - ACS Publications
We have used time-resolved phosphorescence anisotropy (TPA) to probe rotational
dynamics of the rabbit skeletal sarcoplasmic reticulum Ca-ATPase (SERCA), to test the …

[HTML][HTML] Distinct types of abnormality in kinetic properties of three Darier disease-causing sarco (endo) plasmic reticulum Ca2+-ATPase mutants that exhibit normal …

K Sato, K Yamasaki, T Daiho, Y Miyauchi… - Journal of Biological …, 2004 - ASBMB
The possible functional abnormalities in three different Darier disease-causing Ca 2+-
ATPase (SERCA2b) mutants, Ile 274→ Val at the lumenal end of M3, Leu 321→ Phe on the …

The Time-Dependent Distribution of Phosphorylated Intermediates in Native Sarcoplasmic Reticulum Ca2+-ATPase from Skeletal Muscle Is Not Compatible with a …

JE Mahaney, DD Thomas, JP Froehlich - Biochemistry, 2004 - ACS Publications
Quenched-flow mixing was used to characterize the kinetic behavior of the intermediate
reactions of the skeletal muscle sarcoplasmic reticulum (SR) Ca-ATPase (SERCA1) at 2 and …

Structural dynamics of the Ca2+-ATPase of sarcoplasmic reticulum. Temperature profiles of fluorescence polarization and intramolecular energy transfer

I Jona, J Matko, A Martonosi - Biochimica et Biophysica Acta (BBA) …, 1990 - Elsevier
The temperature dependence of fluorescence polarization and Förster-type resonance
energy transfer (FRET) was analyzed in the Ca 2+-ATPase of sarcoplasmic reticulum using …

Characterization of the substrate-induced conformational change of N-iodoacetyl-N′-(5-sulfo-1-naphthyl) ethylenediamine-labeled sarcoplasmic reticulum Ca2+ …

K Kubo, H Suzuki, T Kanazawa - … et Biophysica Acta (BBA)-Protein Structure …, 1990 - Elsevier
Cys-674 of the sarcoplasmic reticulum Ca 2+-ATPase was labeled with N-iodoacetyl-N′-(5-
sulfo-1-naphthyl) ethylenediamine without a loss of the catalytic activity, and changes in the …