Multi-wavelength analytical ultracentrifugation of biopolymer mixtures and interactions
Multi-wavelength analytical ultracentrifugation (MW-AUC) is a recent development made
possible by new analytical ultracentrifuge optical systems. MW-AUC extends the basic …
possible by new analytical ultracentrifuge optical systems. MW-AUC extends the basic …
[HTML][HTML] Structure-function studies reveal ComEA contains an oligomerization domain essential for transformation in gram-positive bacteria
I Ahmed, J Hahn, A Henrickson, FT Khaja… - Nature …, 2022 - nature.com
An essential step in bacterial transformation is the uptake of DNA into the periplasm, across
the thick peptidoglycan cell wall of Gram-positive bacteria, or the outer membrane and thin …
the thick peptidoglycan cell wall of Gram-positive bacteria, or the outer membrane and thin …
Using cryo-EM to uncover mechanisms of bacterial transcriptional regulation
DM Wood, RCJ Dobson… - Biochemical Society …, 2021 - portlandpress.com
Transcription is the principal control point for bacterial gene expression, and it enables a
global cellular response to an intracellular or environmental trigger. Transcriptional …
global cellular response to an intracellular or environmental trigger. Transcriptional …
[HTML][HTML] Determination of mRNA copy number in degradable lipid nanoparticles via density contrast analytical ultracentrifugation
A Bepperling, G Richter - European Biophysics Journal, 2023 - Springer
Lipid nanoparticles as delivery system for mRNA have recently attracted attention to a
broader audience as COVID-19 mRNA vaccines. Their low immunogenicity and capability to …
broader audience as COVID-19 mRNA vaccines. Their low immunogenicity and capability to …
Characterization and quantification of adeno-associated virus capsid-loading states by multi-wavelength analytical ultracentrifugation with UltraScan
Aim: We present multi-wavelength (MW) analytical ultracentrifugation (AUC) methods
offering superior accuracy for adeno-associated virus characterization and quantification …
offering superior accuracy for adeno-associated virus characterization and quantification …
[HTML][HTML] Mechanism of NanR gene repression and allosteric induction of bacterial sialic acid metabolism
Bacteria respond to environmental changes by inducing transcription of some genes and
repressing others. Sialic acids, which coat human cell surfaces, are a nutrient source for …
repressing others. Sialic acids, which coat human cell surfaces, are a nutrient source for …
Comparison of three AUC techniques for the determination of the loading status and capsid titer of AAVs
A Bepperling, J Best - European Biophysics Journal, 2023 - Springer
Due to the rise of adeno-associated viruses (AAVs) as gene therapy delivery vectors,
boundary sedimentation velocity analytical ultracentrifugation (boundary SV-AUC) has been …
boundary sedimentation velocity analytical ultracentrifugation (boundary SV-AUC) has been …
Methods for the Design and Analysis of Analytical Ultracentrifugation Experiments
B Demeler - Current Protocols, 2024 - Wiley Online Library
Analytical ultracentrifugation experiments play an integral role in the solution‐phase
characterization of biological macromolecules and their interactions. This unit discusses the …
characterization of biological macromolecules and their interactions. This unit discusses the …
[HTML][HTML] N-acetylmannosamine-6-phosphate 2-epimerase uses a novel substrate-assisted mechanism to catalyze amino sugar epimerization
There are five known general catalytic mechanisms used by enzymes to catalyze
carbohydrate epimerization. The amino sugar epimerase N-acetylmannosamine-6 …
carbohydrate epimerization. The amino sugar epimerase N-acetylmannosamine-6 …
[HTML][HTML] Analytical ultracentrifugation: still the gold standard that offers multiple solutions
RCJ Dobson, TR Patel - European Biophysics Journal, 2020 - Springer
Understanding the nature of macromolecules and their interactions in solution underpins
many fields, including biology, chemistry and materials science. The 24th International …
many fields, including biology, chemistry and materials science. The 24th International …