Targeted nucleases have provided researchers with the ability to manipulate virtually any
genomic sequence, enabling the facile creation of isogenic cell lines and animal models for …

Conspectus The understanding of gene regulation and the structure and function of the
human genome increased dramatically at the end of the 20th century. Yet the technologies …

Engineered genetic circuits for mammalian cells often require extensive fine-tuning to
perform as intended. We present a robust, general, scalable system, called'Boolean logic …

We describe the development of 'recCas9', an RNA-programmed small serine recombinase
that functions in mammalian cells. We fused a catalytically inactive dCas9 to the catalytic …

The fields of molecular genetics, biotechnology and synthetic biology are demanding ever
more sophisticated molecular tools for programmed precise modification of cell genomic …

Life on Earth is incredibly diverse. Yet, underneath that diversity, there are a number of
constants and highly conserved processes: all life is based on DNA and RNA; the genetic …

Protein engineering is used to generate novel protein folds and assemblages, to impart new
properties and functions onto existing proteins, and to enhance our understanding of …

In site-specific recombination, two short DNA sequences ('sites') are each cut at specific
points in both strands, and the cut ends are rejoined to new partners. The enzymes that …

Genome integration is a powerful tool in both basic and applied biological research.
However, traditional genome integration, which is typically mediated by homologous …

CRISPR-based technologies have become central to genome engineering. However,
CRISPR-based editing strategies are dependent on the repair of DNA breaks via …