Natural proteins are often only slightly more stable in the native state than the denatured
state, and an increase in environmental temperature can easily shift the balance toward …

Therapeutic enzymes are valuable biopharmaceuticals in various biomedical applications.
They have been successfully applied for fibrinolysis, cancer treatment, enzyme replacement …

We report the application of ancestral sequence reconstruction on coronavirus spike protein,
resulting in stable and highly soluble ancestral scaffold antigens (AnSAs). The AnSAs …

As both a computational and an experimental endeavor, ancestral sequence reconstruction
remains a timely and important technique. Modern approaches to conduct ancestral …

Phenylalanine ammonia lyase (PAL) has recently emerged as an important therapeutic
enzyme with several biomedical applications. The enzyme catabolizes l-phenylalanine to …

The rapid growth of sequence databases over the past two decades means that protein
engineers faced with optimizing a protein for any given task will often have immediate …

Lac operon is the standard regulator used to control the orthogonality of T7RNA polymerase
(T7RNAP) and T7 promoter in Escherichia coli BL21 (DE3) strain for protein expression …

Aromatics are valuable bulk or fine chemicals with a myriad of important applications.
Currently, their vast majority is produced from petroleum associated with many negative …

Marine algae are the most abundant resource in the marine environment and are still a
promising source of bioactive compounds including hydrocolloids. This study contributes to …

This study aims to assess for the first time the ability of marine polysaccharides and their
derivatives to stimulate natural defenses in olive tree leaves. Alginates, ALSM, and ALCM …