Single-molecule experiments in biological physics: methods and applications
F Ritort - Journal of Physics: Condensed Matter, 2006 - iopscience.iop.org
I review single-molecule experiments (SMEs) in biological physics. Recent technological
developments have provided the tools to design and build scientific instruments of high …
developments have provided the tools to design and build scientific instruments of high …
Optical tweezers analysis of DNA–protein complexes
Cellular DNA is continuously engaged in a molecular dance with many accessory proteins.
These proteins are responsible for orchestrating the storage, maintenance, and transfer of …
These proteins are responsible for orchestrating the storage, maintenance, and transfer of …
A CRISPR–Cas9-triggered strand displacement amplification method for ultrasensitive DNA detection
Although polymerase chain reaction (PCR) is the most widely used method for DNA
amplification, the requirement of thermocycling limits its non-laboratory applications …
amplification, the requirement of thermocycling limits its non-laboratory applications …
Unraveling the structure of DNA during overstretching by using multicolor, single-molecule fluorescence imaging
Single-molecule manipulation studies have revealed that double-stranded DNA undergoes
a structural transition when subjected to tension. At forces that depend on the attachment …
a structural transition when subjected to tension. At forces that depend on the attachment …
Biophysical characterization of DNA binding from single molecule force measurements
KR Chaurasiya, T Paramanathan, MJ McCauley… - Physics of life …, 2010 - Elsevier
Single molecule force spectroscopy is a powerful method that uses the mechanical
properties of DNA to explore DNA interactions. Here we describe how DNA stretching …
properties of DNA to explore DNA interactions. Here we describe how DNA stretching …
Dynamic structure of T4 gene 32 protein filaments facilitates rapid noncooperative protein dissociation
Bacteriophage T4 gene 32 protein (gp32) is a model single-stranded DNA (ssDNA) binding
protein, essential for DNA replication. gp32 forms cooperative filaments on ssDNA through …
protein, essential for DNA replication. gp32 forms cooperative filaments on ssDNA through …
LINE-1 retrotransposition requires the nucleic acid chaperone activity of the ORF1 protein
SL Martin, M Cruceanu, D Branciforte, PW Li… - Journal of molecular …, 2005 - Elsevier
LINE-1 is a highly successful, non-LTR retrotransposon that has played a leading role in
shaping mammalian genomes. These elements move autonomously through an RNA …
shaping mammalian genomes. These elements move autonomously through an RNA …
Generalized facilitated diffusion model for DNA-binding proteins with search and recognition states
M Bauer, R Metzler - Biophysical journal, 2012 - cell.com
Transcription factors (TFs) such as the lac repressor find their target sequence on DNA at
remarkably high rates. In the established Berg-von Hippel model for this search process, the …
remarkably high rates. In the established Berg-von Hippel model for this search process, the …
Nucleic acid binding and chaperone properties of HIV-1 Gag and nucleocapsid proteins
M Cruceanu, MA Urbaneja, CV Hixson… - Nucleic acids …, 2006 - academic.oup.com
The Gag polyprotein of HIV-1 is essential for retroviral replication and packaging. The
nucleocapsid (NC) protein is the primary region for the interaction of Gag with nucleic acids …
nucleocapsid (NC) protein is the primary region for the interaction of Gag with nucleic acids …
Cisplatin induces loop structures and condensation of single DNA molecules
Structural properties of single λ DNA treated with anti-cancer drug cisplatin were studied
with magnetic tweezers and AFM. Under the effect of low-concentration cisplatin, the DNA …
with magnetic tweezers and AFM. Under the effect of low-concentration cisplatin, the DNA …