Proteome-wide tagging with an H2O2 biosensor reveals highly localized and dynamic redox microenvironments
P Kritsiligkou, K Bosch, TK Shen… - Proceedings of the …, 2023 - National Acad Sciences
Hydrogen peroxide (H2O2) sensing and signaling involves the reversible oxidation of
particular thiols on particular proteins to modulate protein function in a dynamic manner …
particular thiols on particular proteins to modulate protein function in a dynamic manner …
[HTML][HTML] Bioenergetic Shift and Proteomic Signature Induced by Lentiviral-Transduction of GFP-Based Biosensors
S Barakat, Ş Çimen, SM Miri, E Vatandaşlar… - Redox Biology, 2024 - Elsevier
Fluorescent proteins (FPs) stand as pivotal tools extensively employed across diverse
biological research endeavors in various model systems. However, long-standing concerns …
biological research endeavors in various model systems. However, long-standing concerns …
Genetically encoded biosensors unveil neuronal injury dynamics via multichromatic ATP and calcium imaging
When a cell sustains damage, it liberates cytosolic ATP, which can serve as an injury signal,
affecting neighboring cells. This study presents a methodological approach that employs in …
affecting neighboring cells. This study presents a methodological approach that employs in …
[HTML][HTML] Nitric oxide biosensor uncovers diminished ferrous iron-dependency of cultured cells adapted to physiological oxygen levels
Iron is an essential metal for cellular metabolism and signaling, but it has adverse effects in
excess. The physiological consequences of iron deficiency are well established, yet the …
excess. The physiological consequences of iron deficiency are well established, yet the …
Probing subcellular iron availability with genetically encoded nitric oxide biosensors
Cellular iron supply is required for various biochemical processes. Measuring bioavailable
iron in cells aids in obtaining a better understanding of its biochemical activities but is …
iron in cells aids in obtaining a better understanding of its biochemical activities but is …
Visualizing hydrogen peroxide and nitric oxide dynamics in endothelial cells using multispectral imaging under controlled oxygen conditions
The complex interplay between hydrogen peroxide (H 2 O 2) and nitric oxide (NO) in
endothelial cells presents challenges due to technical limitations in simultaneous …
endothelial cells presents challenges due to technical limitations in simultaneous …
Chemogenetic approaches to dissect the role of H2O2 in redox-dependent pathways using genetically encoded biosensors
A Ghaffari Zaki, YC Erdoğan… - Biochemical Society …, 2022 - portlandpress.com
Chemogenetic tools are recombinant enzymes that can be targeted to specific organelles
and tissues. The provision or removal of the enzyme substrate permits control of its …
and tissues. The provision or removal of the enzyme substrate permits control of its …
Visualizing H2O2 and NO in endothelial cells: strategies and pitfalls
The relationship between hydrogen peroxide (H2O2) and nitric oxide (NO) in the
vasculature is multifaceted and remains controversial because the dynamic detection of …
vasculature is multifaceted and remains controversial because the dynamic detection of …
Simultaneous manipulation and imaging of chemogenetically induced hydrogen peroxide in hardly transfectable endothelial cells
E Eroğlu - Cumhuriyet Science Journal, 2022 - acikerisim.medipol.edu.tr
Hydrogen peroxide (H2O2) is a critical signaling molecule in vascular cells, which controls
signaling events, yet it can cause pathological oxidative stress in excess. The lack of …
signaling events, yet it can cause pathological oxidative stress in excess. The lack of …
[HTML][HTML] Multispectral Imaging and Single-Cell Analysis with Genetically Encoded Biosensors Unveil Complex Interactions between Extracellular ATP and Intracellular …
E Eroğlu - Cumhuriyet Science Journal, 2024 - csj.cumhuriyet.edu.tr
The dynamic interplay between extracellular ATP (eATP) and intracellular calcium ([Ca2+] i)
serves as a pivotal signaling axis in cellular physiology, influencing a myriad of cellular …
serves as a pivotal signaling axis in cellular physiology, influencing a myriad of cellular …