The global population of individuals over the age of 65 is growing at an unprecedented rate
and is expected to reach 1.6 billion by 2050. Most older individuals are affected by multiple …

In mammals, DNA methylation in the form of 5-methylcytosine (5mC) can be actively
reversed to unmodified cytosine (C) through TET dioxygenase-mediated oxidation of 5mC to …

Sequencing technologies using nucleotide conversion techniques such as cytosine to
thymine in bisulfite-seq and thymine to cytosine in SLAM seq are powerful tools to explore …

INTRODUCTION Nearly all cells in the human body share the same primary genome
sequence consisting of four nucleotide bases. One of the bases, cytosine, is commonly …

Over 17 and 160 types of chemical modifications have been identified in DNA and RNA,
respectively. The interest in understanding the various biological functions of DNA and RNA …

Mammalian DNA methylation is a critical epigenetic mechanism orchestrating gene
expression networks in many biological processes. However, investigation of the functions …

Bisulfite sequencing has been the gold standard for mapping DNA modifications including 5-
methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) for decades,,–. However, this …

DNA methylation is a key layer of epigenetic regulation. The deposition of methylation marks
relies on the catalytic activity of DNA methyltransferases (DNMTs), and their active removal …

Cytosine methylation is a DNA modification generally associated with transcriptional
silencing. Factors that regulate methylation have been linked to human disease, yet how …

Recent technological advances have made it possible to decode DNA methylomes at single-
base-pair resolution under various physiological conditions. Many aberrant or differentially …