Chromosomal rearrangements are generally thought to accumulate gradually over many
generations. However, DNA sequencing of cancer and congenital disorders uncovered a …

Genome editing has therapeutic potential for treating genetic diseases and cancer.
However, the currently most practicable approaches rely on the generation of DNA double …

▪ Abstract We review old and new insights into the structure of the nuclear envelope and the
components responsible for its dynamic reassembly during mitosis. New information is …

Using Xenopus egg extracts, we have developed a completely soluble system for eukaryotic
chromosomal DNA replication. In the absence of a nuclear envelope, a single, complete …

Nuclear actin regulates transcriptional programmes in a manner dependent on its levels and
polymerisation state. This dynamics is determined by the balance of nucleocytoplasmic …

Using immunodepletion of cyclin E and the inhibitor protein p21WAF/CIP1, we demonstrate
that the cyclin E protein, in association with Cdk2, is required for the elongation phase of …

The nuclear membrane forms a major barrier within the cell, permitting levels of regulation
not found in prokaryotes. The dynamics and diverse functions of the nuclear membrane and …

Humans express three major splicing isoforms of LAP2, a lamin-and chromatin-binding
nuclear protein. LAP2β and γ are integral membrane proteins, whereas α is intranuclear …

Xenopus egg extracts treated with the protein kinase inhibitor 6-dimethylaminopurine (6-
DMAP) are unable to support the initiation of DNA replication. Nuclei assembled in 6-DMAP …

We have analysed the role of topoisomerase II (topo II) in plasmid DNA replication in
Xenopus egg extracts, using specific inhibitors and two-dimensional gel electrophoresis of …