Fluorescent tools have revolutionized our ability to probe biological dynamics, particularly at
the cellular level. Fluorescent sensors have been developed on several platforms, utilizing …

Site-specific modification of proteins is a major challenge in modern chemical biology due to
the large number of reactive functional groups typically present in polypeptides. Because of …

The inverse-electron-demand Diels–Alder cycloaddition between trans-cyclooctenes and
tetrazines is biocompatible and exceptionally fast. We utilized this chemistry for site-specific …

Traditionally, non-specific chemical conjugations, such as acylation of amines on lysine or
alkylation of thiols on cysteines, are widely used; however, they have several shortcomings …

Collagens are fibrous proteins that are integral to the strength and stability of connective
tissues. During collagen maturation, lysyl oxidases (LOX) initiate the cross-linking of fibers …

The superfamily of G protein-coupled receptors (GPCRs) mediates a wide range of
physiological responses and serves as an important category of drug targets. Earlier …

Methods for targeting of small molecules to cellular proteins can allow imaging with
fluorophores that are smaller, brighter, and more photostable than fluorescent proteins …

Live cell imaging brings us into a new era of direct visualization of biological processes and
molecular dynamics in real time. To visualize dynamic cellular processes and virus–host …

This protocol describes an efficient method to site-specifically label cell-surface or purified
proteins with chemical probes in two steps: probe incorporation mediated by enzymes …

Chemical fluorophores offer tremendous size and photophysical advantages over
fluorescent proteins but are much more challenging to target to specific cellular proteins …