CRISPR-Cas systems for editing, regulating and targeting genomes
JD Sander, JK Joung - Nature biotechnology, 2014 - nature.com
Targeted genome editing using engineered nucleases has rapidly gone from being a niche
technology to a mainstream method used by many biological researchers. This widespread …
technology to a mainstream method used by many biological researchers. This widespread …
[HTML][HTML] Small tech, big impact: Agri-nanotechnology journey to optimize crop protection and production for sustainable agriculture
The world's climate shifts rapidly, leading to increasingly severe and volatile weather,
negatively impacting crop yields. To produce long-lasting crops, cutting-edge …
negatively impacting crop yields. To produce long-lasting crops, cutting-edge …
Efficient genome editing in plants using a CRISPR/Cas system
Z Feng, B Zhang, W Ding, X Liu, DL Yang, P Wei… - Cell research, 2013 - nature.com
In the past few years, the development of sequencespecific DNA nucleases has progressed
rapidly and such nucleases have shown their power in generating efficient targeted …
rapidly and such nucleases have shown their power in generating efficient targeted …
A critical review: recent advancements in the use of CRISPR/Cas9 technology to enhance crops and alleviate global food crises
Genome editing (GE) has revolutionized the biological sciences by creating a novel
approach for manipulating the genomes of living organisms. Many tools have been …
approach for manipulating the genomes of living organisms. Many tools have been …
Genome engineering with zinc-finger nucleases
D Carroll - Genetics, 2011 - academic.oup.com
Zinc-finger nucleases (ZFNs) are targetable DNA cleavage reagents that have been
adopted as gene-targeting tools. ZFN-induced double-strand breaks are subject to cellular …
adopted as gene-targeting tools. ZFN-induced double-strand breaks are subject to cellular …
Genome engineering with targetable nucleases
D Carroll - Annual review of biochemistry, 2014 - annualreviews.org
Current technology enables the production of highly specific genome modifications with
excellent efficiency and specificity. Key to this capability are targetable DNA cleavage …
excellent efficiency and specificity. Key to this capability are targetable DNA cleavage …
De novo-engineered transcription activator-like effector (TALE) hybrid nuclease with novel DNA binding specificity creates double-strand breaks
MM Mahfouz, L Li… - Proceedings of the …, 2011 - National Acad Sciences
Site-specific and rare cutting nucleases are valuable tools for genome engineering. The
generation of double-strand DNA breaks (DSBs) promotes homologous recombination in …
generation of double-strand DNA breaks (DSBs) promotes homologous recombination in …
A rapid and general assay for monitoring endogenous gene modification
The development of zinc finger nucleases for targeted gene modification can benefit from
rapid functional assays that directly quantify activity at the endogenous target. Here we …
rapid functional assays that directly quantify activity at the endogenous target. Here we …
Mitochondrially targeted ZFN s for selective degradation of pathogenic mitochondrial genomes bearing large‐scale deletions or point mutations
PA Gammage, J Rorbach, AI Vincent… - EMBO molecular …, 2014 - embopress.org
We designed and engineered mitochondrially targeted obligate heterodimeric zinc finger
nucleases (mt ZFN s) for site‐specific elimination of pathogenic human mitochondrial DNA …
nucleases (mt ZFN s) for site‐specific elimination of pathogenic human mitochondrial DNA …
Targeted gene inactivation in zebrafish using engineered zinc-finger nucleases
Direct genomic manipulation at a specific locus is still not feasible in most vertebrate model
organisms. In vertebrate cell lines, genomic lesions at a specific site have been introduced …
organisms. In vertebrate cell lines, genomic lesions at a specific site have been introduced …