Phage defence by deaminase-mediated depletion of deoxynucleotides in bacteria

BY Hsueh, GB Severin, CA Elg, EJ Waldron… - Nature …, 2022 - nature.com
Abstract Vibrio cholerae biotype El Tor is perpetuating the longest cholera pandemic in
recorded history. The genomic islands VSP-1 and VSP-2 distinguish El Tor from previous …

The SARS-CoV-2 programmed− 1 ribosomal frameshifting element crystal structure solved to 2.09 Å using chaperone-assisted RNA crystallography

C Roman, A Lewicka, D Koirala, NS Li… - ACS Chemical …, 2021 - ACS Publications
The programmed− 1 ribosomal frameshifting element (PFSE) of SARS-CoV-2 is a well
conserved structured RNA found in all coronaviruses' genomes. By adopting a pseudoknot …

Current practice in bicistronic ires reporter use: A systematic review

GGH van den Akker, F Zacchini… - International Journal of …, 2021 - mdpi.com
Bicistronic reporter assays have been instrumental for transgene expression, understanding
of internal ribosomal entry site (IRES) translation, and identification of novel cap …

Methods for the directed evolution of biomolecular interactions

VC Xie, MJ Styles, BC Dickinson - Trends in biochemical sciences, 2022 - cell.com
Noncovalent interactions between biomolecules such as proteins and nucleic acids
coordinate all cellular processes through changes in proximity. Tools that perturb these …

Structure of saguaro cactus virus 3′ translational enhancer mimics 5′ cap for eIF4E binding

M Ojha, J Vogt, NK Das, E Redmond… - Proceedings of the …, 2024 - National Acad Sciences
The genomes of several plant viruses contain RNA structures at their 3′ ends called cap-
independent translation enhancers (CITEs) that bind the host protein factors such as mRNA …

Target-dependent RNA polymerase as universal platform for gene expression control in response to intracellular molecules

S Komatsu, H Ohno, H Saito - Nature Communications, 2023 - nature.com
Controlling gene expression in response to specific molecules is an essential technique for
regulating cellular functions. However, current platforms with transcription and translation …

Structural basis for fluorescence activation by Pepper RNA

HC Rees, W Gogacz, NS Li, D Koirala… - ACS chemical …, 2022 - ACS Publications
Pepper is a fluorogenic RNA aptamer tag that binds to a variety of benzylidene-cyanophenyl
(HBC) derivatives with tight affinity and activates their fluorescence. To investigate how …

Synthetic antibodies for accelerated RNA crystallography

S DasGupta - Wiley Interdisciplinary Reviews: RNA, 2024 - Wiley Online Library
RNA structure is crucial to a wide range of cellular processes. The intimate relationship
between macromolecular structure and function necessitates the determination of high …

Structure and function of type IV IRES in picornaviruses: a systematic review

Y Li, L Zhang, L Wang, J Li, Y Zhao, F Liu… - Frontiers in …, 2024 - frontiersin.org
The Picornaviridae is a family of icosahedral viruses with single-stranded, highly diverse
positive-sense RNA genomes. Virions consist of a capsid, without envelope, surrounding a …

Antiviral Effect of Ginsenoside Rb2 and Rb3 Against Bovine Viral Diarrhea Virus and Classical Swine Fever Virus in vitro

B Tan, M Giangaspero, N Sun, Y Jin, K Liu… - Frontiers in Veterinary …, 2021 - frontiersin.org
Bovine viral diarrhea virus (BVDV) and classical swine fever virus (CSFV) are members of
the genus Pestivirus that cause disease in wild and domestic animals and are responsible …