The spatiotemporal fluorescence imaging of biological processes requires effective tools to
label intracellular biomolecules in living systems. This review presents a brief overview of …

Background Intracellular pH underlies most cellular processes. There is emerging evidence
of a pH-signaling role in plant cells and microorganisms. Dysregulation of pH is associated …

A review with 636 references. The literature on saturated amino sulfonic acids with
primary/secondary amino group and their derivatives from XIX century to 2016 is surveyed …

Our ability to observe biochemical events with high spatial and temporal resolution is
essential for understanding the functioning of living systems. Intrinsically fluorescent proteins …

Highlights•Protein–dye complexes that activate fluorescence allow no-wash protein
labeling.•Quencher removal, environmental change and direct binding can activate …

The human immunodeficiency virus type 1 (HIV-1) envelope glycoprotein (Env) encodes
specific trafficking signals within its long cytoplasmic tail (CT) that regulate incorporation into …

Since their discovery, fluorescent probes have found widespread use in biological research.
Over time, multiple next-generation probes increased the fluorescence catalog by offering …

Neuropeptides control rhythmic behaviors, but the timing and location of their release within
circuits is unknown. Here, imaging in the brain shows that synaptic neuropeptide release by …

The pursuit of quantitative biological information via imaging requires robust labeling
approaches that can be used in multiple applications and with a variety of detectable colors …

The development and function of tissues, blood, and the immune system is dependent upon
proximity for cellular recognition and communication. However, the detection of cell-to-cell …