Photobleaching of organic fluorophores: quantitative characterization, mechanisms, protection

AP Demchenko - Methods and applications in fluorescence, 2020 - iopscience.iop.org
Photochemical stability is one of the most important parameters that determine the
usefulness of organic dyes in different applications. This Review addresses key factors that …

The swollen polymer network hypothesis: Quantitative models of hydrogel swelling, stiffness, and solute transport

NR Richbourg, NA Peppas - Progress in Polymer Science, 2020 - Elsevier
The introduction of the work Makromoleküle by Herman Staudinger in 1919 brings back an
understanding of the very early days of polymer science when characterizing the molecular …

Molecular rotors: fluorescent sensors for microviscosity and conformation of biomolecules

M Paez‐Perez, MK Kuimova - Angewandte Chemie, 2024 - Wiley Online Library
The viscosity and crowding of biological environment are considered vital for the correct
cellular function, and alterations in these parameters are known to underly a number of …

Engineering the dynamics of cell adhesion cues in supramolecular hydrogels for facile control over cell encapsulation and behavior

M Diba, S Spaans, SIS Hendrikse… - Advanced …, 2021 - Wiley Online Library
The extracellular matrix (ECM) forms through hierarchical assembly of small and larger
polymeric molecules into a transient, hydrogel‐like fibrous network that provides mechanical …

Catalytic activation of bioorthogonal chemistry with light (CABL) enables rapid, spatiotemporally controlled labeling and no-wash, subcellular 3D-patterning in live …

A Jemas, Y Xie, JE Pigga, JL Caplan… - Journal of the …, 2022 - ACS Publications
Described is the spatiotemporally controlled labeling and patterning of biomolecules in live
cells through the catalytic activation of bioorthogonal chemistry with light, referred to as …

Relevance and regulation of cell density

GE Neurohr, A Amon - Trends in cell biology, 2020 - cell.com
Cell density shows very little variation within a given cell type. For example, in humans
variability in cell density among cells of a given cell type is 100 times smaller than variation …

Methods for physical characterization of phase-separated bodies and membrane-less organelles

DM Mitrea, B Chandra, MC Ferrolino, EB Gibbs… - Journal of molecular …, 2018 - Elsevier
Membrane-less organelles are cellular structures which arise through the phenomenon of
phase separation. This process enables compartmentalization of specific sets of …

The mucus barrier to inhaled gene therapy

GA Duncan, J Jung, J Hanes, JS Suk - Molecular Therapy, 2016 - cell.com
Recent evidence suggests that the airway mucus gel layer may be impermeable to the viral
and synthetic gene vectors used in past inhaled gene therapy clinical trials for diseases like …

Dependence of diffusion in Escherichia coli cytoplasm on protein size, environmental conditions, and cell growth

N Bellotto, J Agudo-Canalejo, R Colin, R Golestanian… - Elife, 2022 - elifesciences.org
Inside prokaryotic cells, passive translational diffusion typically limits the rates with which
cytoplasmic proteins can reach their locations. Diffusion is thus fundamental to most cellular …

Novel Biological Functions of ZIF‐NP as a Delivery Vehicle: High Pulmonary Accumulation, Favorable Biocompatibility, and Improved Therapeutic Outcome

S Li, K Wang, Y Shi, Y Cui, B Chen, B He… - Advanced Functional …, 2016 - Wiley Online Library
Though zeolitic imidazole framework (ZIF) emerges as an advanced functional material for
small‐molecule delivery due to its unique features such as high loading and pH‐sensitive …