An important part of characterizing any protein molecule is to determine its size and shape.
Sedimentation and gel filtration are hydrodynamic techniques that can be used for this …

Analytical ultracentrifugation (AU) is reemerging as a versatile tool for the study of proteins.
Monitoring the sedimentation of macromolecules in the centrifugal field allows their …

Analytical ultracentrifugation (AUC) is a versatile and powerful method for the quantitative
analysis of macromolecules in solution. AUC has broad applications for the study of …

The ubiquitous purine nucleoside phosphorylases (PNPs) play a key role in the purine
salvage pathway, and PNP deficiency in humans leads to an impairment of T-cell function …

LIR-1 is a class I MHC receptor related to natural killer inhibitory receptors (KIRs). Binding of
LIR-1 or KIRs to class I molecules results in inhibitory signals. Unlike individual KIRs, LIR-1 …

▪ Abstract Analytical ultracentrifugation is a classical method of biochemistry and molecular
biology. Because it is a primary technique, sedimentation can provide first-principle …

In this postgenomic era, the ability to identify protein–protein interactions on a genomic scale
is very important to assist in the assignment of physiological function. Because of the …

Regularly spaced nucleosomal arrays equilibrate between unfolded and highly folded
conformations in< 2 mM MgCl2, and self-associate above 2 mM MgCl2 [Schwarz, PM, & …

Analytical ultracentrifugation (AUC) and field flow fractionation (FFF) are 2 important
biophysical methods for measuring protein aggregates. Both methods can separate protein …

Analytical ultracentrifugation methods were utilized to further characterize the acid
denaturation pathways of wild-type, V30M, and L55P transthyretin (TTR) that generate …