Deubiquitinases: From mechanisms to their inhibition by small molecules

SM Lange, LA Armstrong, Y Kulathu - Molecular cell, 2022 - cell.com
Deubiquitinases (DUBs) are specialized proteases that remove ubiquitin from substrates or
cleave within ubiquitin chains to regulate ubiquitylation and therefore play important roles in …

Resolving the complexity of ubiquitin networks

K Kliza, K Husnjak - Frontiers in molecular biosciences, 2020 - frontiersin.org
Ubiquitination regulates nearly all cellular processes by coordinated activity of ubiquitin
writers (E1, E2, and E3 enzymes), erasers (deubiquitinating enzymes) and readers (proteins …

BioE3 identifies specific substrates of ubiquitin E3 ligases

O Barroso-Gomila, L Merino-Cacho, V Muratore… - Nature …, 2023 - nature.com
Hundreds of E3 ligases play a critical role in recognizing specific substrates for modification
by ubiquitin (Ub). Separating genuine targets of E3s from E3-interactors remains a …

[HTML][HTML] MINDY-1 is a member of an evolutionarily conserved and structurally distinct new family of deubiquitinating enzymes

SAA Rehman, YA Kristariyanto, SY Choi, PJ Nkosi… - Molecular cell, 2016 - cell.com
Deubiquitinating enzymes (DUBs) remove ubiquitin (Ub) from Ub-conjugated substrates to
regulate the functional outcome of ubiquitylation. Here we report the discovery of a new …

Discovery and characterization of ZUFSP/ZUP1, a distinct deubiquitinase class important for genome stability

D Kwasna, SAA Rehman, J Natarajan, S Matthews… - Molecular Cell, 2018 - cell.com
Deubiquitinating enzymes (DUBs) are important regulators of ubiquitin signaling. Here, we
report the discovery of deubiquitinating activity in ZUFSP/C6orf113. High-resolution crystal …

[HTML][HTML] Site-specific ubiquitylation and SUMOylation using genetic-code expansion and sortase

M Fottner, AD Brunner, V Bittl, D Horn-Ghetko… - Nature chemical …, 2019 - nature.com
Post-translational modification of proteins with ubiquitin and ubiquitin-like proteins (Ubls) is
central to the regulation of eukaryotic cellular processes. Our ability to study the effects of …

Organization and dynamics of the nonhomologous end-joining machinery during DNA double-strand break repair

DA Reid, S Keegan, A Leo-Macias… - Proceedings of the …, 2015 - National Acad Sciences
Nonhomologous end-joining (NHEJ) is a major repair pathway for DNA double-strand
breaks (DSBs), involving synapsis and ligation of the broken strands. We describe the use of …

Systems‐wide analysis of BCR signalosomes and downstream phosphorylation and ubiquitylation

S Satpathy, SA Wagner, P Beli, R Gupta… - Molecular systems …, 2015 - embopress.org
B‐cell receptor (BCR) signaling is essential for the development and function of B cells;
however, the spectrum of proteins involved in BCR signaling is not fully known. Here we …

Genetic Encoding of Cyanopyridylalanine for In‐Cell Protein Macrocyclization by the Nitrile–Aminothiol Click Reaction

EH Abdelkader, H Qianzhu, J George… - Angewandte …, 2022 - Wiley Online Library
Cyanopyridylalanines are non‐canonical amino acids that react with aminothiol compounds
under physiological conditions in a biocompatible manner without requiring added catalyst …

UBE2N promotes melanoma growth via MEK/FRA1/SOX10 signaling

A Dikshit, YJ Jin, S Degan, J Hwang, MW Foster, CY Li… - Cancer research, 2018 - AACR
Abstract UBE2N is a K63-specific ubiquitin conjugase linked to various immune disorders
and cancer. Here, we demonstrate that UBE2N and its partners UBE2V1 and UBE2V2 are …