Enzymes as catalysts in synthetic organic chemistry gained importance in the latter half of
the 20th century, but nevertheless suffered from two major limitations. First, many enzymes …

Recent developments in computational chemistry and biology have come together in the
“inside‐out” approach to enzyme engineering. Proteins have been designed to catalyze …

Asymmetric catalysis plays a key role in modern synthetic organic chemistry, with synthetic
catalysts and enzymes being the two available options. During the latter part of the last …

Enzymes have the potential to catalyse a wide variety of chemical reactions. They are
increasingly being sought as environmentally friendly and cost‐effective alternatives to …

Biocatalysis is an enabling technology adding to organic oxidation chemistry. Especially the
high selectivity of enzymatic oxidation coevally operating under mild conditions and not …

Directed evolution can generate a remarkable range of new enzyme properties. Alternate
substrate specificities and reaction selectivities are readily accessible in enzymes from …

Efficacy in laboratory evolution of enzymes is currently a pressing issue, making
comparative studies of different methods and strategies mandatory. Recent reports indicate …

Engineering artificial enzymes with high activity and catalytic mechanism different from
naturally occurring enzymes is a challenge in protein design. For example, many attempts …

Additive or non‐additive, that is the question: The increasing awareness of non‐additive
cooperative mutational effects in protein engineering is currently providing new theoretical …

Unspecific peroxygenases (UPOs) perform oxyfunctionalizations for a wide range of
substrates utilizing H2O2 without the need for further reductive equivalents or electron …