dPCR: a technology review

PL Quan, M Sauzade, E Brouzes - Sensors, 2018 - mdpi.com
Digital Polymerase Chain Reaction (dPCR) is a novel method for the absolute quantification
of target nucleic acids. Quantification by dPCR hinges on the fact that the random …

Droplet digital PCR of viral‎ DNA/RNA, current progress, challenges, and future perspectives

AA Kojabad, M Farzanehpour… - Journal of Medical …, 2021 - Wiley Online Library
High‐throughput droplet‐based digital PCR (ddPCR) is a refinement of the conventional
polymerase chain reaction (PCR)‎ methods. In ddPCR, DNA/RNA is encapsulated …

The digital MIQE guidelines update: minimum information for publication of quantitative digital PCR experiments for 2020

JF Huggett - Clinical chemistry, 2020 - academic.oup.com
Digital PCR (dPCR) has developed considerably since the publication of the Minimum
Information for Publication of Digital PCR Experiments (dMIQE) guidelines in 2013, with …

The Digital MIQE Guidelines: Minimum Information for Publication of Quantitative Digital PCR Experiments

JF Huggett, CA Foy, V Benes, K Emslie… - Clinical …, 2013 - academic.oup.com
There is growing interest in digital PCR (dPCR) because technological progress makes it a
practical and increasingly affordable technology. dPCR allows the precise quantification of …

Digital assays part I: partitioning statistics and digital PCR

AS Basu - SLAS TECHNOLOGY: Translating Life Sciences …, 2017 - journals.sagepub.com
A digital assay is one in which the sample is partitioned into many small containers such that
each partition contains a discrete number of biological entities (0, 1, 2, 3,…). A powerful …

Considerations for digital PCR as an accurate molecular diagnostic tool

JF Huggett, S Cowen, CA Foy - Clinical chemistry, 2015 - academic.oup.com
Abstract BACKGROUND Digital PCR (dPCR) is an increasingly popular manifestation of
PCR that offers a number of unique advantages when applied to preclinical research …

Antiretroviral-free HIV-1 remission and viral rebound after allogeneic stem cell transplantation: report of 2 cases

TJ Henrich, E Hanhauser, FM Marty… - Annals of internal …, 2014 - acpjournals.org
Background: It is unknown whether the reduction in HIV-1 reservoirs seen after allogeneic
hematopoietic stem cell transplantation (HSCT) with susceptible donor cells is sufficient to …

Applications of digital PCR for clinical microbiology

J Kuypers, KR Jerome - Journal of clinical microbiology, 2017 - Am Soc Microbiol
Digital PCR (dPCR) is an important new tool for use in the clinical microbiology laboratory.
Its advantages over quantitative PCR (qPCR), including absolute quantification without a …

Defective proviruses rapidly accumulate during acute HIV-1 infection

KM Bruner, AJ Murray, RA Pollack, MG Soliman… - Nature medicine, 2016 - nature.com
Although antiretroviral therapy (ART) suppresses viral replication to clinically undetectable
levels, human immunodeficiency virus type 1 (HIV-1) persists in CD4+ T cells in a latent form …

Current commercial dPCR platforms: Technology and market review

LL Tan, N Loganathan, S Agarwalla… - Critical Reviews in …, 2023 - Taylor & Francis
Digital polymerase chain reaction (dPCR) technology has provided a new technique for
molecular diagnostics, with superior advantages, such as higher sensitivity, precision, and …