Alternative splicing as a source of phenotypic diversity
A major goal of evolutionary genetics is to understand the genetic processes that give rise to
phenotypic diversity in multicellular organisms. Alternative splicing generates multiple …
phenotypic diversity in multicellular organisms. Alternative splicing generates multiple …
From words to complete phrases: insight into single-cell isoforms using short and long reads
The profiling of gene expression patterns to glean biological insights from single cells has
become commonplace over the last few years. However, this approach overlooks the …
become commonplace over the last few years. However, this approach overlooks the …
Single-cell long-read sequencing-based mapping reveals specialized splicing patterns in developing and adult mouse and human brain
RNA isoforms influence cell identity and function. However, a comprehensive brain isoform
map was lacking. We analyze single-cell RNA isoforms across brain regions, cell subtypes …
map was lacking. We analyze single-cell RNA isoforms across brain regions, cell subtypes …
Systematic analysis of alternative exon-dependent interactome remodeling reveals multitasking functions of gene regulatory factors
Alternative splicing significantly expands biological complexity, particularly in the vertebrate
nervous system. Increasing evidence indicates that developmental and tissue-dependent …
nervous system. Increasing evidence indicates that developmental and tissue-dependent …
[HTML][HTML] Single-cell long-read mRNA isoform regulation is pervasive across mammalian brain regions, cell types, and development
RNA isoforms influence cell identity and function. Until recently, technological limitations
prevented a genome-wide appraisal of isoform influence on cell identity in various parts of …
prevented a genome-wide appraisal of isoform influence on cell identity in various parts of …
Unsupervised reference-free inference reveals unrecognized regulated transcriptomic complexity in human single cells
Myriad mechanisms diversify the sequence content of eukaryotic transcripts at the DNA and
RNA level with profound functional consequences. Examples include diversity generated by …
RNA level with profound functional consequences. Examples include diversity generated by …
Interrogations of single-cell RNA splicing landscapes with SCASL define new cell identities with physiological relevance
X Xiang, Y He, Z Zhang, X Yang - Nature Communications, 2024 - nature.com
RNA splicing shapes the gene regulatory programs that underlie various physiological and
disease processes. Here, we present the SCASL (single-cell clustering based on alternative …
disease processes. Here, we present the SCASL (single-cell clustering based on alternative …
A statistical, reference-free algorithm subsumes myriad problems in genome science and enables novel discovery
We present a unifying statistical formulation for many fundamental problems in genome
science and develop a reference-free, highly efficient algorithm that solves it. Sequence …
science and develop a reference-free, highly efficient algorithm that solves it. Sequence …
Investigating RNA splicing as a source of cellular diversity using a binomial mixture model
K Isaev, DA Knowles - Machine Learning in Computational …, 2024 - proceedings.mlr.press
Alternative splicing (AS) contributes significantly to RNA and protein variability yet its role in
defining cellular diversity is not fully understood. While Smart-seq2 offers enhanced …
defining cellular diversity is not fully understood. While Smart-seq2 offers enhanced …
Deep Transcriptomics Reveals Cell-Specific Isoforms of Pan-Neuronal Genes
Profiling gene expression in single neurons using single-cell RNA-Seq is a powerful method
for understanding the molecular diversity of the nervous system. Profiling alternative splicing …
for understanding the molecular diversity of the nervous system. Profiling alternative splicing …