[HTML][HTML] Expression and purification of recombinant G protein-coupled receptors: A review
DN Wiseman, A Otchere, JH Patel, R Uddin… - Protein Expression and …, 2020 - Elsevier
Given their extensive role in cell signalling, GPCRs are significant drug targets; despite this,
many of these receptors have limited or no available prophylaxis. Novel drug design and …
many of these receptors have limited or no available prophylaxis. Novel drug design and …
Baculovirus as a gene delivery vector: recent understandings of molecular alterations in transduced cells and latest applications
Baculovirus infects insects in nature and is non-pathogenic to humans, but can transduce a
broad range of mammalian and avian cells. Thanks to the biosafety, large cloning capacity …
broad range of mammalian and avian cells. Thanks to the biosafety, large cloning capacity …
Expression of recombinant proteins in insect and mammalian cells
EA McKenzie, WM Abbott - Methods, 2018 - Elsevier
Purified recombinant proteins are key reagents in academic and industrial research. The
ability to make these proteins quickly often relies on the availability of higher eukaryotic cell …
ability to make these proteins quickly often relies on the availability of higher eukaryotic cell …
Recent advances in the production of proteins in insect and mammalian cells for structural biology
JE Nettleship, R Assenberg, JM Diprose… - Journal of structural …, 2010 - Elsevier
The production of proteins in sufficient quantity and of appropriate quality is an essential pre-
requisite for structural studies. Escherichia coli remains the dominant expression system in …
requisite for structural studies. Escherichia coli remains the dominant expression system in …
Fundamentals of baculovirus expression and applications
TA Kost, CW Kemp - … technologies for protein complex production and …, 2016 - Springer
In 1982 E. coli produced human insulin, the world's first recombinant DNA drug, was
approved by the FDA. Since this historical event, remarkable progress has been made in …
approved by the FDA. Since this historical event, remarkable progress has been made in …
Rapid titration of measles and other viruses: optimization with determination of replication cycle length
Background Measles virus (MV) is a member of the Paramyxoviridae family and an
important human pathogen causing strong immunosuppression in affected individuals and a …
important human pathogen causing strong immunosuppression in affected individuals and a …
Bioprocessing of baculovirus vectors: a review
MG Aucoin, JA Mena, AA Kamen - Current gene therapy, 2010 - ingentaconnect.com
The baculovirus/insect cell expression system is best known, and used, as a research tool
for the production of recombinant proteins often requiring post-translational modifications …
for the production of recombinant proteins often requiring post-translational modifications …
Enabling PAT in insect cell bioprocesses: In situ monitoring of recombinant adeno‐associated virus production by fluorescence spectroscopy
The process analytical technology (PAT) initiative shifted the bioprocess development
mindset towards real‐time monitoring and control tools to measure relevant process …
mindset towards real‐time monitoring and control tools to measure relevant process …
Multivariate data analysis in cell gene therapy manufacturing
J Emerson, B Kara, J Glassey - Biotechnology Advances, 2020 - Elsevier
The emergence of cell gene therapy (CGT) as a safe and efficacious treatment for numerous
severe inherited and acquired human diseases has led to growing interest and investment …
severe inherited and acquired human diseases has led to growing interest and investment …
Purification of influenza virus‐like particles using sulfated cellulose membrane adsorbers
ABSTRACT BACKGROUND Vaccines based on virus‐like particles (VLPs) are an
alternative to inactivated viral vaccines that combine good safety profiles with strong …
alternative to inactivated viral vaccines that combine good safety profiles with strong …