New advances in cross-linking mass spectrometry toward structural systems biology

C Yu, L Huang - Current opinion in chemical biology, 2023 - Elsevier
Elucidating protein–protein interaction (PPI) networks and their structural features within
cells is central to understanding fundamental biology and associations of cell phenotypes …

Residue selective crosslinking of proteins through photoactivatable or proximity-enabled reactivity

J Liu, B Yang, L Wang - Current opinion in chemical biology, 2023 - Elsevier
Photo-and chemical crosslinking of proteins have offered various avenues for studying
protein structure and protein interactions with biomolecules. Conventional photoactivatable …

Dissecting diazirine photo-reaction mechanism for protein residue-specific cross-linking and distance mapping

Y Jiang, X Zhang, H Nie, J Fan, S Di, H Fu… - Nature …, 2024 - nature.com
While photo-cross-linking (PXL) with alkyl diazirines can provide stringent distance restraints
and offer insights into protein structures, unambiguous identification of cross-linked residues …

Mass Spectrometry Structural Proteomics Enabled by Limited Proteolysis and Cross‐Linking

H Lu, Z Zhu, L Fields, H Zhang… - Mass Spectrometry …, 2024 - Wiley Online Library
The exploration of protein structure and function stands at the forefront of life science and
represents an ever‐expanding focus in the development of proteomics. As mass …

ComMap: a software to perform large-scale structure-based mapping for cross-linking mass spectrometry

W Zhang, Y Shan, L Zhao, Z Liang, C Liu… - …, 2023 - academic.oup.com
Motivation Chemical cross-linking combined with mass spectrometry (CXMS) is now a well-
established method for profiling existing protein–protein interactions (PPIs) with partially …