A field guide to whole‐genome sequencing, assembly and annotation
Genome sequencing projects were long confined to biomedical model organisms and
required the concerted effort of large consortia. Rapid progress in high‐throughput …
required the concerted effort of large consortia. Rapid progress in high‐throughput …
Sequencing technologies—the next generation
ML Metzker - Nature reviews genetics, 2010 - nature.com
Demand has never been greater for revolutionary technologies that deliver fast, inexpensive
and accurate genome information. This challenge has catalysed the development of next …
and accurate genome information. This challenge has catalysed the development of next …
NOVOPlasty: de novo assembly of organelle genomes from whole genome data
N Dierckxsens, P Mardulyn, G Smits - Nucleic acids research, 2017 - academic.oup.com
The evolution in next-generation sequencing (NGS) technology has led to the development
of many different assembly algorithms, but few of them focus on assembling the organelle …
of many different assembly algorithms, but few of them focus on assembling the organelle …
The cityscapes dataset for semantic urban scene understanding
Visual understanding of complex urban street scenes is an enabling factor for a wide range
of applications. Object detection has benefited enormously from large-scale datasets …
of applications. Object detection has benefited enormously from large-scale datasets …
Fusobacterium nucleatum infection is prevalent in human colorectal carcinoma
M Castellarin, RL Warren, JD Freeman… - Genome …, 2012 - genome.cshlp.org
An estimated 15% or more of the cancer burden worldwide is attributable to known
infectious agents. We screened colorectal carcinoma and matched normal tissue specimens …
infectious agents. We screened colorectal carcinoma and matched normal tissue specimens …
Scaffolding pre-assembled contigs using SSPACE
De novo assembly tools play a main role in reconstructing genomes from next-generation
sequencing (NGS) data and usually yield a number of contigs. Using paired-read …
sequencing (NGS) data and usually yield a number of contigs. Using paired-read …
De novo assembly of human genomes with massively parallel short read sequencing
Next-generation massively parallel DNA sequencing technologies provide ultrahigh
throughput at a substantially lower unit data cost; however, the data are very short read …
throughput at a substantially lower unit data cost; however, the data are very short read …
The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR Experiments
Background: Currently, a lack of consensus exists on how best to perform and interpret
quantitative real-time PCR (qPCR) experiments. The problem is exacerbated by a lack of …
quantitative real-time PCR (qPCR) experiments. The problem is exacerbated by a lack of …
ABySS: a parallel assembler for short read sequence data
Widespread adoption of massively parallel deoxyribonucleic acid (DNA) sequencing
instruments has prompted the recent development of de novo short read assembly …
instruments has prompted the recent development of de novo short read assembly …
Next-generation DNA sequencing
J Shendure, H Ji - Nature biotechnology, 2008 - nature.com
DNA sequence represents a single format onto which a broad range of biological
phenomena can be projected for high-throughput data collection. Over the past three years …
phenomena can be projected for high-throughput data collection. Over the past three years …