Bioprocess development today is slow and expensive compared to chemical process
development. A drastic paradigm shift is necessary and possible by the consistent …

A decade ago, a team of biochemists including two of us, modeled yeast glycolysis and
showed that one of the most studied biochemical pathways could not be quite understood in …

In vitro enzymatic assays of cell-free extracts offer an opportunity to assess in vivo enzyme
concentrations. If performed under conditions that resemble the conditions in vivo, they may …

A macroscopic model describing the influence of nitrogen on a fed-batch baker's yeast
production process is proposed. First, on the basis of a set of biological reactions, inspired …

Due to its vital importance in the supply of cellular pathways with energy and precursors,
glycolysis has been studied for several decades regarding its capacity and regulation. For a …

Qualitative phenotypic changes are the integrated result of quantitative changes at multiple
regulatory levels. To explain the temperature-induced increase of glycolytic flux in …

Enzymology tends to focus on highly specific effects of substrates, allosteric modifiers, and
products occurring at low concentrations, because these are most informative about the …

Regulation analysis is a methodology that quantifies to what extent a change in the flux
through a metabolic pathway is regulated by either gene expression or metabolism. Two …

The genome-wide transcriptional response of Saccharomyces cerevisiae to a temperature
shift from 28° C to 41° C–a temperature at which cell viability was not impaired, but growth …

The aim of this work was to further investigate the glycolysis performance of lager and ale
brewer's yeasts under different fermentation temperature using a combined analysis of …