Virus-like particles: preparation, immunogenicity and their roles as nanovaccines and drug nanocarriers

S Nooraei, H Bahrulolum, ZS Hoseini… - Journal of …, 2021 - Springer
Virus-like particles (VLPs) are virus-derived structures made up of one or more different
molecules with the ability to self-assemble, mimicking the form and size of a virus particle but …

Cell-free gene expression

D Garenne, MC Haines, EF Romantseva… - Nature reviews …, 2021 - nature.com
Cell-free gene expression (CFE) emerged as an alternative approach to living cells for
specific applications in protein synthesis and labelling for structural biology and proteomics …

A user's guide to cell-free protein synthesis

NE Gregorio, MZ Levine, JP Oza - Methods and protocols, 2019 - mdpi.com
Cell-free protein synthesis (CFPS) is a platform technology that provides new opportunities
for protein expression, metabolic engineering, therapeutic development, education, and …

Virus‐like particles: Next‐generation nanoparticles for targeted therapeutic delivery

MJ Rohovie, M Nagasawa… - … & translational medicine, 2017 - Wiley Online Library
Most drug therapies distribute the agents throughout the entire body, even though the drugs
are typically only needed at specific tissues. This often limits dosage and causes discomfort …

Protein nanoparticles: promising platforms for drug delivery applications

A Jain, SK Singh, SK Arya, SC Kundu… - … Biomaterials Science & …, 2018 - ACS Publications
The development of drug delivery systems using nanoparticles as carriers for small and
large therapeutic molecules remains a rapidly growing area of research. The advantages of …

High-throughput preparation methods of crude extract for robust cell-free protein synthesis

YC Kwon, MC Jewett - Scientific reports, 2015 - nature.com
Crude extract based cell-free protein synthesis (CFPS) has emerged as a powerful
technology platform for high-throughput protein production and genetic part characterization …

Cell-free protein synthesis: applications come of age

ED Carlson, R Gan, CE Hodgman, MC Jewett - Biotechnology advances, 2012 - Elsevier
Cell-free protein synthesis has emerged as a powerful technology platform to help satisfy the
growing demand for simple and efficient protein production. While used for decades as a …

Construction and characterization of virus-like particles: a review

A Zeltins - Molecular biotechnology, 2013 - Springer
Over the last three decades, virus-like particles (VLPs) have evolved to become a widely
accepted technology, especially in the field of vaccinology. In fact, some VLP-based …

Bioengineering virus‐like particles as vaccines

LHL Lua, NK Connors, F Sainsbury… - Biotechnology and …, 2014 - Wiley Online Library
Virus‐like particle (VLP) technology seeks to harness the optimally tuned immunostimulatory
properties of natural viruses while omitting the infectious trait. VLPs that assemble from a …

Exploring the potential of cell-free protein synthesis for extending the abilities of biological systems

K Khambhati, G Bhattacharjee, N Gohil… - … in bioengineering and …, 2019 - frontiersin.org
Cell-free protein synthesis (CFPS) system is a simple, rapid, and sensitive tool that is devoid
of membrane-bound barriers, yet contains all the mandatory substrates, biomolecules, and …