This review summarizes our current understanding of translation in prokaryotes, focusing on
the mechanistic and structural aspects of each phase of translation: initiation, elongation …

In this review, we highlight the current understanding of translation elongation and recoding
in eukaryotes. In addition to providing an overview of the process, recent advances in our …

The quality of diffraction data obtained using silver and molybdenum microsources has been
compared for six model compounds with a wide range of absorption factors. The …

Translation factor eIF5A, containing the unique amino acid hypusine, was originally shown
to stimulate Met-puromycin synthesis, a model assay for peptide bond formation. More …

The high-resolution structures of ribosomal subunits published in 2000 have revolutionized
the field of protein translation. They facilitated the determination and interpretation of …

We describe two structures of the intact bacterial ribosome from Escherichia coli determined
to a resolution of 3.5 angstroms by x-ray crystallography. These structures provide a detailed …

Many clinically useful antibiotics exert their antimicrobial effects by blocking protein
synthesis on the bacterial ribosome. The structure of the ribosome has recently been …

Translation initiation is a major rate-limiting step for protein synthesis. However, recent
studies strongly suggest that the efficiency of protein synthesis is additionally regulated by …

During peptide-bond formation on the ribosome, the α-amine of an aminoacyl-tRNA attacks
the ester carbonyl carbon of a peptidyl-tRNA to yield a peptide lengthened by one amino …

Ribosomes, which are central to protein synthesis and convert transcribed mRNAs into
polypeptide chains, have been the focus of structural and biochemical studies for more than …