Ion mobility mass spectrometry (IM-MS) for structural biology: insights gained by measuring mass, charge, and collision cross section

E Christofi, P Barran - Chemical Reviews, 2023 - ACS Publications
The investigation of macromolecular biomolecules with ion mobility mass spectrometry (IM-
MS) techniques has provided substantial insights into the field of structural biology over the …

Adding a new separation dimension to MS and LC–MS: what is the utility of ion mobility spectrometry?

V d'Atri, T Causon, O Hernandez‐Alba… - Journal of separation …, 2018 - Wiley Online Library
Ion mobility spectrometry is an analytical technique known for more than 100 years, which
entails separating ions in the gas phase based on their size, shape, and charge. While ion …

Mass spectrometry based tools to investigate protein–ligand interactions for drug discovery

KJ Pacholarz, RA Garlish, RJ Taylor… - Chemical Society …, 2012 - pubs.rsc.org
The initial stages of drug discovery are increasingly reliant on development and
improvement of analytical methods to investigate protein–protein and protein–ligand …

Visible and ultraviolet spectroscopy of gas phase protein ions

R Antoine, P Dugourd - Physical Chemistry Chemical Physics, 2011 - pubs.rsc.org
Optical spectroscopy has contributed enormously to our knowledge of the structure and
dynamics of atoms and molecules and is now emerging as a cornerstone of the gas phase …

Unique insights to intrinsically disordered proteins provided by ion mobility mass spectrometry

D Stuchfield, P Barran - Current opinion in chemical biology, 2018 - Elsevier
Entire functional proteins as well as large regions of proteins lack structural elements which
are resolvable via crystallography or NMR. These intrinsically disordered proteins (IDPs) or …

Mass spectrometry methods for intrinsically disordered proteins

R Beveridge, Q Chappuis, C Macphee, P Barran - Analyst, 2013 - pubs.rsc.org
In the last ten years mass spectrometry has emerged as a powerful biophysical technique
capable of providing unique insights into the structure and dynamics of proteins. Part of this …

Intrinsically disordered p53 and its complexes populate compact conformations in the gas phase

K Pagel, E Natan, Z Hall, AR Fersht… - Angewandte Chemie …, 2013 - pure.mpg.de
Spontaneous shrinking: The intrinsically disordered tumor suppressor protein p53 was
analyzed by using a combination of ion mobility mass spectrometry and molecular dynamics …

Folding of a salivary intrinsically disordered protein upon binding to tannins

F Canon, R Ballivian, F Chirot, R Antoine… - Journal of the …, 2011 - ACS Publications
We used ion mobility spectrometry to explore conformational adaptability of intrinsically
disordered proteins bound to their targets in complex mixtures. We investigated the …

Electron transfer with no dissociation ion mobility–mass spectrometry (ETnoD IM-MS). The effect of charge reduction on protein conformation

JR Jhingree, R Beveridge, ER Dickinson… - International Journal of …, 2017 - Elsevier
A novel mass spectrometry approach is reported which investigate how ion-molecule charge
reduction reactions between radical anions and protein cations modulate protein …

Intrinsic disorder in proteins: a challenge for (un) structural biology met by ion mobility–mass spectrometry

E Jurneczko, F Cruickshank, M Porrini… - Biochemical Society …, 2012 - portlandpress.com
The link between structure and function of a given protein is a principal tenet of biology. The
established approach to understand the function of a protein is to 'solve'its structure and …