Selected reaction monitoring–based proteomics: workflows, potential, pitfalls and future directions
P Picotti, R Aebersold - Nature methods, 2012 - nature.com
Selected reaction monitoring (SRM) is a targeted mass spectrometry technique that is
emerging in the field of proteomics as a complement to untargeted shotgun methods. SRM is …
emerging in the field of proteomics as a complement to untargeted shotgun methods. SRM is …
[HTML][HTML] Mass spectrometry based biomarker discovery, verification, and validation–quality assurance and control of protein biomarker assays
CE Parker, CH Borchers - Molecular oncology, 2014 - Elsevier
In its early years, mass spectrometry (MS)-based proteomics focused on the cataloging of
proteins found in different species or different tissues. By 2005, proteomics was being used …
proteins found in different species or different tissues. By 2005, proteomics was being used …
Precision of heavy–light peptide ratios measured by MALDI-TOF mass spectrometry
NL Anderson, M Razavi, TW Pearson… - Journal of proteome …, 2012 - ACS Publications
We have investigated the precision of peptide quantitation by MALDI-TOF mass
spectrometry (MS) using six pairs of proteotypic peptides (light) and same-sequence stable …
spectrometry (MS) using six pairs of proteotypic peptides (light) and same-sequence stable …
A specific function for the histone chaperone NASP to fine-tune a reservoir of soluble H3-H4 in the histone supply chain
Proper genome packaging requires coordination of both DNA and histone metabolism.
While histone gene transcription and RNA processing adequately provide for scheduled …
While histone gene transcription and RNA processing adequately provide for scheduled …
Prevention of amino acid conversion in SILAC experiments with embryonic stem cells
Recent studies using stable isotope labeling with amino acids in culture (SILAC) in
quantitative proteomics have made mention of the problematic conversion of isotope-coded …
quantitative proteomics have made mention of the problematic conversion of isotope-coded …
Current affairs in quantitative targeted proteomics: multiple reaction monitoring–mass spectrometry
AK Yocum, AM Chinnaiyan - Briefings in Functional Genomics …, 2009 - academic.oup.com
Quantitative targeted proteomics has recently taken front stage in the proteomics community.
Centered on multiple reaction monitoring–mass spectrometry (MRM–MS) methodologies …
Centered on multiple reaction monitoring–mass spectrometry (MRM–MS) methodologies …
Proteome of human stem cells from periodontal ligament and dental pulp
E Eleuterio, O Trubiani, M Sulpizio, F Di Giuseppe… - PLoS …, 2013 - journals.plos.org
Background Many adult tissues contain a population of stem cells with the ability to
regenerate structures similar to the microenvironments from which they are derived in vivo …
regenerate structures similar to the microenvironments from which they are derived in vivo …
Human embryonic mesodermal progenitors highly resemble human mesenchymal stem cells and display high potential for tissue engineering applications
GM De Peppo, S Svensson, M Lennerås… - … Engineering Part A, 2010 - liebertpub.com
Adult stem cells, such as human mesenchymal stem cells (hMSCs), show limited
proliferative capacity and, after long-term culture, lose their differentiation capacity and are …
proliferative capacity and, after long-term culture, lose their differentiation capacity and are …
Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC) and Quantitative Comparison of the Membrane Proteomes of Self-renewing and Differentiating Human …
TA Prokhorova, KTG Rigbolt, PT Johansen… - Molecular & Cellular …, 2009 - ASBMB
Stable isotope labeling by amino acids in cell culture (SILAC) is a powerful quantitative
proteomics platform for comprehensive characterization of complex biological systems …
proteomics platform for comprehensive characterization of complex biological systems …
Targeted proteomics by selected reaction monitoring mass spectrometry: applications to systems biology and biomarker discovery
S Elschenbroich, T Kislinger - Molecular bioSystems, 2011 - pubs.rsc.org
Mass Spectrometry-based proteomics is now considered a relatively established strategy for
protein analysis, ranging from global expression profiling to the identification of protein …
protein analysis, ranging from global expression profiling to the identification of protein …