[HTML][HTML] SYNCAS: Efficient CRISPR/Cas9 gene-editing in difficult to transform arthropods

S De Rouck, A Mocchetti, W Dermauw… - Insect Biochemistry and …, 2024 - Elsevier
The genome editing technique CRISPR/Cas9 has led to major advancements in many
research fields and this state-of-the-art tool has proven its use in genetic studies for various …

Biotechnology-enhanced genetic controls of the global pest Drosophila suzukii

Y Yan, HMM Ahmed, EA Wimmer, MF Schetelig - Trends in Biotechnology, 2024 - cell.com
Abstract Spotted wing Drosophila (Drosophila suzukii Matsumura, or SWD), an insect pest of
soft-skinned fruits native to East Asia, has rapidly spread worldwide in the past 15 years …

Gene drive and genetic sex conversion in the global agricultural pest Ceratitis capitata

A Meccariello, S Hou, S Davydova, JD Fawcett… - Nature …, 2024 - nature.com
Homing-based gene drives are recently proposed interventions promising the area-wide,
species-specific genetic control of harmful insect populations. Here we characterise a first …

AY chromosome-linked genome editor for efficient population suppression in the malaria vector Anopheles gambiae

I Tolosana, K Willis, M Gribble, L Phillimore… - Nature …, 2025 - nature.com
Genetic control–the deliberate introduction of genetic traits to control a pest or vector
population–offers a powerful tool to augment conventional mosquito control tools that have …

Developing genetic tools to control the Oriental fruit fly: Potential approaches and target genes

N Traoré, R Galizi, M Kientega, H Maïga… - Journal of Applied …, 2024 - Wiley Online Library
Abstract The Oriental fruit fly, Bactrocera dorsalis, is a pest of significant economic interest
that damages horticulture and causes major economic losses worldwide. The main control …

Population suppression by release of insects carrying a dominant sterile homing gene drive targeting doublesex in Drosophila

W Chen, J Guo, Y Liu, J Champer - Nature Communications, 2024 - nature.com
CRISPR homing gene drives can suppress pest populations by targeting female fertility
genes, converting wild-type alleles into drive alleles in the germline of drive heterozygotes …

Improving the suppressive power of homing gene drive by co-targeting a distant-site female fertility gene

NR Faber, X Xu, J Chen, S Hou, J Du… - Nature …, 2024 - nature.com
Gene drive technology has the potential to address major biological challenges. Well-
studied homing suppression drives have been shown to be highly efficient in Anopheles …

[HTML][HTML] SYNCAS based CRISPR-Cas9 gene editing in predatory mites, whiteflies and stinkbugs

A Mocchetti, S De Rouck, S Naessens… - Insect Biochemistry and …, 2025 - Elsevier
Despite the establishment of CRISPR-Cas9 gene editing protocols in a wide range of
organisms, genetic engineering is still challenging for many organisms due to constraints …

Population suppression with dominant female-lethal alleles is boosted by homing gene drive

J Zhu, J Chen, Y Liu, X Xu, J Champer - BMC biology, 2024 - Springer
Background Methods to suppress pest insect populations using genetic constructs and
repeated releases of male homozygotes have recently been shown to be an attractive …

Germline Cas9 promoters with improved performance for homing gene drive

J Du, W Chen, X Jia, X Xu, E Yang, R Zhou… - Nature …, 2024 - nature.com
Gene drive systems could be a viable strategy to prevent pathogen transmission or suppress
vector populations by propagating drive alleles with super-Mendelian inheritance. CRISPR …