Selection of reference genes for the normalization of RT-qPCR data in gene expression studies in insects: a systematic review
Reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR) is a reliable
technique for quantifying expression levels of targeted genes during various biological …
technique for quantifying expression levels of targeted genes during various biological …
Gene expression studies of reference genes for quantitative real-time PCR: an overview in insects
Whenever gene expression is being examined, it is essential that a normalization process is
carried out to eliminate non-biological variations. The use of reference genes, such as …
carried out to eliminate non-biological variations. The use of reference genes, such as …
Identification and Validation of Reference Genes for Gene Expression Analysis Using Quantitative PCR in Spodoptera litura (Lepidoptera: Noctuidae)
Y Lu, M Yuan, X Gao, T Kang, S Zhan, H Wan, J Li - PloS one, 2013 - journals.plos.org
Reverse transcription quantitative polymerase chain reaction (qRT-PCR) has rapidly
become the most sensitive and accurate method for the quantification of gene expression …
become the most sensitive and accurate method for the quantification of gene expression …
Validation of reference genes for expression analysis by quantitative real-time PCR in Leptinotarsa decemlineata (Say)
XQ Shi, WC Guo, PJ Wan, LT Zhou, XL Ren… - BMC research …, 2013 - Springer
Background L. decemlineata is an exotic invasive insect pest, and invaded in Xinjiang Uygur
autonomous region in China in the 1990s from Kazakhstan. It is a notorious defoliator of …
autonomous region in China in the 1990s from Kazakhstan. It is a notorious defoliator of …
RNA-Seq and molecular docking reveal multi-level pesticide resistance in the bed bug
Abstract Background Bed bugs (Cimex lectularius) are hematophagous nocturnal parasites
of humans that have attained high impact status due to their worldwide resurgence. The …
of humans that have attained high impact status due to their worldwide resurgence. The …
Validation of Reference Genes in Solenopsis invicta in Different Developmental Stages, Castes and Tissues
D Cheng, Z Zhang, X He, G Liang - PLoS one, 2013 - journals.plos.org
To accurately assess gene expression levels, it is essential to normalize real-time
quantitative PCR (RT-qPCR) data with suitable internal reference genes. For the red …
quantitative PCR (RT-qPCR) data with suitable internal reference genes. For the red …
Evaluation of Housekeeping Genes for Quantitative Real-Time PCR Analysis of Bradysia odoriphaga (Diptera: Sciaridae)
C Shi, F Yang, X Zhu, E Du, Y Yang, S Wang… - International Journal of …, 2016 - mdpi.com
The soil insect Bradysia odoriphaga (Diptera: Sciaridae) causes substantial damage to
Chinese chive. Suitable reference genes in B. odoriphaga (Bradysia odoriphaga) have yet …
Chinese chive. Suitable reference genes in B. odoriphaga (Bradysia odoriphaga) have yet …
Reference Genes for Expression Analyses by qRT-PCR in Phthorimaea operculella (Lepidoptera: Gelechiidae)
CH Shen, LJ Peng, YX Zhang, HR Zeng, HF Yu, L Jin… - Insects, 2022 - mdpi.com
Simple Summary Quantitative real-time fluorescent polymerase chain reaction (qRT-PCR) is
a momentous tool for calculating the expression levels of targeted genes across various …
a momentous tool for calculating the expression levels of targeted genes across various …
Identification and Validation of Reference Genes for Quantitative Real-Time PCR in Drosophila suzukii (Diptera: Drosophilidae)
Y Zhai, Q Lin, X Zhou, X Zhang, T Liu, Y Yu - PLoS One, 2014 - journals.plos.org
To accurately evaluate gene expression levels and obtain more accurate quantitative real-
time RT-PCR (qRT-PCR) data, normalization relative to reliable reference gene (s) is …
time RT-PCR (qRT-PCR) data, normalization relative to reliable reference gene (s) is …
Selection of housekeeping genes and demonstration of RNAi in cotton leafhopper, Amrasca biguttula biguttula (Ishida)
Amrasca biguttula biguttula (Ishida) commonly known as cotton leafhopper is a severe pest
of cotton and okra. Not much is known on this insect at molecular level due to lack of …
of cotton and okra. Not much is known on this insect at molecular level due to lack of …