Selection of reference genes for the normalization of RT-qPCR data in gene expression studies in insects: a systematic review

J Lü, C Yang, Y Zhang, H Pan - Frontiers in physiology, 2018 - frontiersin.org
Reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR) is a reliable
technique for quantifying expression levels of targeted genes during various biological …

Gene expression studies of reference genes for quantitative real-time PCR: an overview in insects

M Shakeel, A Rodriguez, UB Tahir, F Jin - Biotechnology letters, 2018 - Springer
Whenever gene expression is being examined, it is essential that a normalization process is
carried out to eliminate non-biological variations. The use of reference genes, such as …

Identification and Validation of Reference Genes for Gene Expression Analysis Using Quantitative PCR in Spodoptera litura (Lepidoptera: Noctuidae)

Y Lu, M Yuan, X Gao, T Kang, S Zhan, H Wan, J Li - PloS one, 2013 - journals.plos.org
Reverse transcription quantitative polymerase chain reaction (qRT-PCR) has rapidly
become the most sensitive and accurate method for the quantification of gene expression …

Validation of reference genes for expression analysis by quantitative real-time PCR in Leptinotarsa decemlineata (Say)

XQ Shi, WC Guo, PJ Wan, LT Zhou, XL Ren… - BMC research …, 2013 - Springer
Background L. decemlineata is an exotic invasive insect pest, and invaded in Xinjiang Uygur
autonomous region in China in the 1990s from Kazakhstan. It is a notorious defoliator of …

RNA-Seq and molecular docking reveal multi-level pesticide resistance in the bed bug

P Mamidala, AJ Wijeratne, S Wijeratne, K Kornacker… - BMC genomics, 2012 - Springer
Abstract Background Bed bugs (Cimex lectularius) are hematophagous nocturnal parasites
of humans that have attained high impact status due to their worldwide resurgence. The …

Validation of Reference Genes in Solenopsis invicta in Different Developmental Stages, Castes and Tissues

D Cheng, Z Zhang, X He, G Liang - PLoS one, 2013 - journals.plos.org
To accurately assess gene expression levels, it is essential to normalize real-time
quantitative PCR (RT-qPCR) data with suitable internal reference genes. For the red …

Evaluation of Housekeeping Genes for Quantitative Real-Time PCR Analysis of Bradysia odoriphaga (Diptera: Sciaridae)

C Shi, F Yang, X Zhu, E Du, Y Yang, S Wang… - International Journal of …, 2016 - mdpi.com
The soil insect Bradysia odoriphaga (Diptera: Sciaridae) causes substantial damage to
Chinese chive. Suitable reference genes in B. odoriphaga (Bradysia odoriphaga) have yet …

Reference Genes for Expression Analyses by qRT-PCR in Phthorimaea operculella (Lepidoptera: Gelechiidae)

CH Shen, LJ Peng, YX Zhang, HR Zeng, HF Yu, L Jin… - Insects, 2022 - mdpi.com
Simple Summary Quantitative real-time fluorescent polymerase chain reaction (qRT-PCR) is
a momentous tool for calculating the expression levels of targeted genes across various …

Identification and Validation of Reference Genes for Quantitative Real-Time PCR in Drosophila suzukii (Diptera: Drosophilidae)

Y Zhai, Q Lin, X Zhou, X Zhang, T Liu, Y Yu - PLoS One, 2014 - journals.plos.org
To accurately evaluate gene expression levels and obtain more accurate quantitative real-
time RT-PCR (qRT-PCR) data, normalization relative to reliable reference gene (s) is …

Selection of housekeeping genes and demonstration of RNAi in cotton leafhopper, Amrasca biguttula biguttula (Ishida)

S Singh, M Gupta, S Pandher, G Kaur, P Rathore… - Plos one, 2018 - journals.plos.org
Amrasca biguttula biguttula (Ishida) commonly known as cotton leafhopper is a severe pest
of cotton and okra. Not much is known on this insect at molecular level due to lack of …