Methods in virus diagnostics: from ELISA to next generation sequencing

N Boonham, J Kreuze, S Winter, R van der Vlugt… - Virus research, 2014 - Elsevier
Despite the seemingly continuous development of newer and ever more elaborate methods
for detecting and identifying viruses, very few of these new methods get adopted for routine …

Cassava brown streak disease: a threat to food security in Africa

BL Patil, JP Legg, E Kanju… - Journal of General …, 2015 - microbiologyresearch.org
Cassava brown streak disease (CBSD) has emerged as the most important viral disease of
cassava (Manihot esculenta) in Africa and is a major threat to food security. CBSD is caused …

Simultaneous CRISPR/Cas9‐mediated editing of cassava eIF4E isoforms nCBP‐1 and nCBP‐2 reduces cassava brown streak disease symptom severity and …

MA Gomez, ZD Lin, T Moll, RD Chauhan… - Plant biotechnology …, 2019 - Wiley Online Library
Cassava brown streak disease (CBSD) is a major constraint on cassava yields in East and
Central Africa and threatens production in West Africa. CBSD is caused by two species of …

Cassava virus diseases: biology, epidemiology, and management

JP Legg, PL Kumar, T Makeshkumar, L Tripathi… - Advances in virus …, 2015 - Elsevier
Abstract Cassava (Manihot esculenta Crantz.) is the most important vegetatively propagated
food staple in Africa and a prominent industrial crop in Latin America and Asia. Its vegetative …

Use of next‐generation sequencing for the identification and characterization of Maize chlorotic mottle virus and Sugarcane mosaic virus causing maize lethal …

IP Adams, DW Miano, ZM Kinyua, A Wangai… - Plant …, 2013 - Wiley Online Library
The diagnosis of novel unidentified viral plant diseases can be problematic, as the
conventional methods such as real‐time PCR or ELISA may be too specific to a particular …

Cassava brown streak disease: historical timeline, current knowledge and future prospects

KR Tomlinson, AM Bailey, T Alicai… - Molecular Plant …, 2018 - Wiley Online Library
Cassava is the second most important staple food crop in terms of per capita calories
consumed in Africa and holds potential for climate change adaptation. Unfortunately …

Accuracy of a smartphone-based object detection model, PlantVillage Nuru, in identifying the foliar symptoms of the viral diseases of cassava–CMD and CBSD

LM Mrisho, NA Mbilinyi, M Ndalahwa… - Frontiers in plant …, 2020 - frontiersin.org
Nuru is a deep learning object detection model for diagnosing plant diseases and pests
developed as a public good by PlantVillage (Penn State University), FAO, IITA, CIMMYT …

Field evaluation of selected cassava genotypes for cassava brown streak disease based on symptom expression and virus load

T Kaweesi, R Kawuki, V Kyaligonza, Y Baguma… - Virology journal, 2014 - Springer
Abstract Background Production of cassava (Manihot esculenta Crantz), a food security crop
in sub-Saharan Africa, is threatened by the spread of cassava brown streak disease (CBSD) …

Transcriptional response of virus-infected cassava and identification of putative sources of resistance for cassava brown streak disease

MN Maruthi, S Bouvaine, HA Tufan, IU Mohammed… - PloS one, 2014 - journals.plos.org
Cassava (Manihot esculenta) is a major food staple in sub-Saharan Africa, which is severely
affected by cassava brown streak disease (CBSD). The aim of this study was to identify …

Early detection of plant virus infection using multispectral imaging and spatial–spectral machine learning

Y Peng, MM Dallas, JT Ascencio-Ibáñez, JS Hoyer… - Scientific Reports, 2022 - nature.com
Cassava brown streak disease (CBSD) is an emerging viral disease that can greatly reduce
cassava productivity, while causing only mild aerial symptoms that develop late in infection …