Successful evolutionary enzyme engineering requires a high throughput screening or
selection method, which considerably increases the chance of obtaining desired properties …

The key practical consideration in directed evolution of functional biomolecules is the
linkage of genotype and phenotype. In vitro selections offer the potential to select from …

The mRNA display approach to in vitro protein selection is based upon the puromycin‐
mediated formation of a covalent bond between an mRNA and its gene product. This …

A method for selective and comprehensive enrichment of N-linked glycopeptides was
developed to facilitate detection of micro-heterogeneity of N-glycosylation. The method takes …

We have assembled references of 700 articles published in 2001 that describe work
performed using commercially available optical biosensors. To illustrate the technology's …

In vitro compartmentalization (IVC) uses water-in-oil emulsions to create artificial cell-like
compartments in which genes can be individually transcribed and translated. Here, we …

Cell penetrating peptides (CPPs) have tremendous potential for use in gene and drug
delivery applications. The selection of new CPPs with desired capabilities from randomized …

Gene expression technologies where nucleic acid sequences remain physically linked to
their corresponding gene products are important tools for selection and identification of rare …

The DNA ligase from bacteriophage T4 is one of the most widely used enzymes in molecular
biology. It has evolved to seal single-stranded nicks in double-stranded DNA, but not to join …

Generating functional protein variants with novel or improved characteristics has been a
goal of the biotechnology industry and life sciences, for decades. Rational design and …