Applications of environmental DNA (eDNA) in ecology and conservation: opportunities, challenges and prospects

KC Beng, RT Corlett - Biodiversity and conservation, 2020 - Springer
Conserving biodiversity in the face of ever-increasing human pressure is hampered by our
lack of basic information on species occurrence, distribution, abundance, habitat …

[HTML][HTML] DNA barcode reference libraries for the monitoring of aquatic biota in Europe: Gap-analysis and recommendations for future work

H Weigand, AJ Beermann, F Čiampor, FO Costa… - Science of the Total …, 2019 - Elsevier
Effective identification of species using short DNA fragments (DNA barcoding and DNA
metabarcoding) requires reliable sequence reference libraries of known taxa. Both …

Biases in bulk: DNA metabarcoding of marine communities and the methodology involved

LM van der Loos, R Nijland - Molecular Ecology, 2021 - Wiley Online Library
With the growing anthropogenic pressure on marine ecosystems, the need for efficient
monitoring of biodiversity grows stronger. DNA metabarcoding of bulk samples is …

Assessment of BOLD and GenBank–Their accuracy and reliability for the identification of biological materials

KA Meiklejohn, N Damaso, JM Robertson - PloS one, 2019 - journals.plos.org
Taxonomic identification of biological materials can be achieved through DNA barcoding,
where an unknown “barcode” sequence is compared to a reference database. In many …

Prospects and challenges of implementing DNA metabarcoding for high-throughput insect surveillance

AM Piper, J Batovska, NOI Cogan, J Weiss… - …, 2019 - academic.oup.com
Trap-based surveillance strategies are widely used for monitoring of invasive insect species,
aiming to detect newly arrived exotic taxa as well as track the population levels of …

Validation of COI metabarcoding primers for terrestrial arthropods

V Elbrecht, TWA Braukmann, NV Ivanova, SWJ Prosser… - PeerJ, 2019 - peerj.com
Metabarcoding can rapidly determine the species composition of bulk samples and thus
aids biodiversity and ecosystem assessment. However, it is essential to use primer sets that …

GenBank is a reliable resource for 21st century biodiversity research

M Leray, N Knowlton, SL Ho… - Proceedings of the …, 2019 - National Acad Sciences
Traditional methods of characterizing biodiversity are increasingly being supplemented and
replaced by approaches based on DNA sequencing alone. These approaches commonly …

Metabarcoding a diverse arthropod mock community

TWA Braukmann, NV Ivanova… - Molecular ecology …, 2019 - Wiley Online Library
Although DNA metabarcoding is an attractive approach for monitoring biodiversity, it is often
difficult to detect all the species present in a bulk sample. In particular, sequence recovery …

Harnessing the power of eDNA metabarcoding for the detection of deep-sea fishes

B McClenaghan, N Fahner, D Cote, J Chawarski… - PloS one, 2020 - journals.plos.org
The deep ocean is the largest biome on Earth and faces increasing anthropogenic
pressures from climate change and commercial fisheries. Our ability to sustainably manage …

COI metabarcoding primer choice affects richness and recovery of indicator taxa in freshwater systems

M Hajibabaei, TM Porter, M Wright, J Rudar - PLoS One, 2019 - journals.plos.org
Mixed community or environmental DNA marker gene sequencing has become a commonly
used technique for biodiversity analyses in freshwater systems. Many cytochrome c oxidase …