Single-molecule localization microscopy

M Lelek, MT Gyparaki, G Beliu, F Schueder… - Nature reviews …, 2021 - nature.com
Single-molecule localization microscopy (SMLM) describes a family of powerful imaging
techniques that dramatically improve spatial resolution over standard, diffraction-limited …

Super-resolution microscopy for structural cell biology

S Liu, P Hoess, J Ries - Annual review of biophysics, 2022 - annualreviews.org
Super-resolution microscopy techniques, and specifically single-molecule localization
microscopy (SMLM), are approaching nanometer resolution inside cells and thus have great …

Super-resolution microscopy with single molecules in biology and beyond–essentials, current trends, and future challenges

L Möckl, WE Moerner - Journal of the American Chemical …, 2020 - ACS Publications
Single-molecule super-resolution microscopy has developed from a specialized technique
into one of the most versatile and powerful imaging methods of the nanoscale over the past …

Switchable fluorophores for single-molecule localization microscopy

H Li, JC Vaughan - Chemical reviews, 2018 - ACS Publications
The past decade has witnessed an explosion in the use of super-resolution fluorescence
microscopy methods in biology and other fields. Single-molecule localization microscopy …

Single-molecule fluorescence microscopy review: shedding new light on old problems

S Shashkova, MC Leake - Bioscience reports, 2017 - portlandpress.com
Fluorescence microscopy is an invaluable tool in the biosciences, a genuine workhorse
technique offering exceptional contrast in conjunction with high specificity of labelling with …

MINSTED nanoscopy enters the Ångström localization range

M Weber, H von der Emde, M Leutenegger… - Nature …, 2023 - nature.com
Super-resolution techniques have achieved localization precisions in the nanometer regime.
Here we report all-optical, room temperature localization of fluorophores with precision in …

Localization microscopy at doubled precision with patterned illumination

J Cnossen, T Hinsdale, RØ Thorsen, M Siemons… - Nature …, 2020 - nature.com
MINFLUX offers a breakthrough in single molecule localization precision, but is limited in
field of view. Here we combine centroid estimation and illumination pattern induced photon …

Cryogenic super-resolution fluorescence and electron microscopy correlated at the nanoscale

PD Dahlberg, WE Moerner - Annual Review of Physical …, 2021 - annualreviews.org
We review the emerging method of super-resolved cryogenic correlative light and electron
microscopy (srCryoCLEM). Super-resolution (SR) fluorescence microscopy and cryogenic …

Bleaching‐Resistant Super‐Resolution Fluorescence Microscopy

J Kwon, MS Elgawish, SH Shim - Advanced Science, 2022 - Wiley Online Library
Photobleaching is the permanent loss of fluorescence after extended exposure to light and
is a major limiting factor in super‐resolution microscopy (SRM) that restricts spatiotemporal …

Diversity in sensing and signaling of bacterial sensor histidine kinases

E Ishii, Y Eguchi - Biomolecules, 2021 - mdpi.com
Two-component signal transduction systems (TCSs) are widely conserved in bacteria to
respond to and adapt to the changing environment. Since TCSs are also involved in …