CRISPR technology: A decade of genome editing is only the beginning

JY Wang, JA Doudna - Science, 2023 - science.org
The advent of clustered regularly interspaced short palindromic repeat (CRISPR) genome
editing, coupled with advances in computing and imaging capabilities, has initiated a new …

Applications of CRISPR–Cas in agriculture and plant biotechnology

H Zhu, C Li, C Gao - Nature Reviews Molecular Cell Biology, 2020 - nature.com
The prokaryote-derived CRISPR–Cas genome editing technology has altered plant
molecular biology beyond all expectations. Characterized by robustness and high target …

Heritable transgene-free genome editing in plants by grafting of wild-type shoots to transgenic donor rootstocks

L Yang, F Machin, S Wang, E Saplaoura… - Nature …, 2023 - nature.com
Generation of stable gene-edited plant lines using clustered regularly interspaced short
palindromic repeats (CRISPR)–CRISPR-associated protein 9 (Cas9) requires a lengthy …

Genome editing of a rice CDP-DAG synthase confers multipathogen resistance

G Sha, P Sun, X Kong, X Han, Q Sun, L Fouillen… - Nature, 2023 - nature.com
The discovery and application of genome editing introduced a new era of plant breeding by
giving researchers efficient tools for the precise engineering of crop genomes. Here we …

Broad-spectrum resistance to bacterial blight in rice using genome editing

R Oliva, C Ji, G Atienza-Grande, JC Huguet-Tapia… - Nature …, 2019 - nature.com
Bacterial blight of rice is an important disease in Asia and Africa. The pathogen,
Xanthomonas oryzae pv. oryzae (Xoo), secretes one or more of six known transcription …

CRISPR/Cas genome editing and precision plant breeding in agriculture

K Chen, Y Wang, R Zhang, H Zhang… - Annual review of plant …, 2019 - annualreviews.org
Enhanced agricultural production through innovative breeding technology is urgently
needed to increase access to nutritious foods worldwide. Recent advances in CRISPR/Cas …

Multiplexed CRISPR technologies for gene editing and transcriptional regulation

NS McCarty, AE Graham, L Studená… - Nature …, 2020 - nature.com
Multiplexed CRISPR technologies, in which numerous gRNAs or Cas enzymes are
expressed at once, have facilitated powerful biological engineering applications, vastly …

Highly efficient DNA-free plant genome editing using virally delivered CRISPR–Cas9

X Ma, X Zhang, H Liu, Z Li - Nature Plants, 2020 - nature.com
Genome-editing technologies using CRISPR–Cas nucleases have revolutionized plant
science and hold enormous promise in crop improvement. Conventional transgene …

The emerging and uncultivated potential of CRISPR technology in plant science

Y Zhang, AA Malzahn, S Sretenovic, Y Qi - Nature Plants, 2019 - nature.com
The application of clustered regularly interspaced short palindromic repeats (CRISPR) for
genetic manipulation has revolutionized life science over the past few years. CRISPR was …

Expanded base editing in rice and wheat using a Cas9-adenosine deaminase fusion

C Li, Y Zong, Y Wang, S Jin, D Zhang, Q Song… - Genome biology, 2018 - Springer
Nucleotide base editors in plants have been limited to conversion of cytosine to thymine.
Here, we describe a new plant adenine base editor based on an evolved tRNA adenosine …