CRISPR technology: A decade of genome editing is only the beginning
JY Wang, JA Doudna - Science, 2023 - science.org
The advent of clustered regularly interspaced short palindromic repeat (CRISPR) genome
editing, coupled with advances in computing and imaging capabilities, has initiated a new …
editing, coupled with advances in computing and imaging capabilities, has initiated a new …
Applications of CRISPR–Cas in agriculture and plant biotechnology
The prokaryote-derived CRISPR–Cas genome editing technology has altered plant
molecular biology beyond all expectations. Characterized by robustness and high target …
molecular biology beyond all expectations. Characterized by robustness and high target …
Heritable transgene-free genome editing in plants by grafting of wild-type shoots to transgenic donor rootstocks
Generation of stable gene-edited plant lines using clustered regularly interspaced short
palindromic repeats (CRISPR)–CRISPR-associated protein 9 (Cas9) requires a lengthy …
palindromic repeats (CRISPR)–CRISPR-associated protein 9 (Cas9) requires a lengthy …
Genome editing of a rice CDP-DAG synthase confers multipathogen resistance
G Sha, P Sun, X Kong, X Han, Q Sun, L Fouillen… - Nature, 2023 - nature.com
The discovery and application of genome editing introduced a new era of plant breeding by
giving researchers efficient tools for the precise engineering of crop genomes. Here we …
giving researchers efficient tools for the precise engineering of crop genomes. Here we …
Broad-spectrum resistance to bacterial blight in rice using genome editing
R Oliva, C Ji, G Atienza-Grande, JC Huguet-Tapia… - Nature …, 2019 - nature.com
Bacterial blight of rice is an important disease in Asia and Africa. The pathogen,
Xanthomonas oryzae pv. oryzae (Xoo), secretes one or more of six known transcription …
Xanthomonas oryzae pv. oryzae (Xoo), secretes one or more of six known transcription …
CRISPR/Cas genome editing and precision plant breeding in agriculture
K Chen, Y Wang, R Zhang, H Zhang… - Annual review of plant …, 2019 - annualreviews.org
Enhanced agricultural production through innovative breeding technology is urgently
needed to increase access to nutritious foods worldwide. Recent advances in CRISPR/Cas …
needed to increase access to nutritious foods worldwide. Recent advances in CRISPR/Cas …
Multiplexed CRISPR technologies for gene editing and transcriptional regulation
NS McCarty, AE Graham, L Studená… - Nature …, 2020 - nature.com
Multiplexed CRISPR technologies, in which numerous gRNAs or Cas enzymes are
expressed at once, have facilitated powerful biological engineering applications, vastly …
expressed at once, have facilitated powerful biological engineering applications, vastly …
Highly efficient DNA-free plant genome editing using virally delivered CRISPR–Cas9
X Ma, X Zhang, H Liu, Z Li - Nature Plants, 2020 - nature.com
Genome-editing technologies using CRISPR–Cas nucleases have revolutionized plant
science and hold enormous promise in crop improvement. Conventional transgene …
science and hold enormous promise in crop improvement. Conventional transgene …
The emerging and uncultivated potential of CRISPR technology in plant science
The application of clustered regularly interspaced short palindromic repeats (CRISPR) for
genetic manipulation has revolutionized life science over the past few years. CRISPR was …
genetic manipulation has revolutionized life science over the past few years. CRISPR was …
Expanded base editing in rice and wheat using a Cas9-adenosine deaminase fusion
Nucleotide base editors in plants have been limited to conversion of cytosine to thymine.
Here, we describe a new plant adenine base editor based on an evolved tRNA adenosine …
Here, we describe a new plant adenine base editor based on an evolved tRNA adenosine …