Protein semisynthesis—defined herein as the assembly of a protein from a combination of
synthetic and recombinant fragments—is a burgeoning field of chemical biology that has …

▪ Abstract Expressed protein ligation (EPL) is a protein engineering approach that allows
recombinant and synthetic polypeptides to be chemoselectively and regioselectively joined …

Inteins are auto-processing domains found in organisms from all domains of life. These
proteins carry out a process known as protein splicing, which is a multi-step biochemical …

Regulation of protein function through thiol-based redox switches plays an important role in
the response and adaptation to local and global changes in the cellular levels of reactive …

Inteins are self-catalytic protein splicing elements. InBase (http://www. neb. com/neb/inteins.
html), the Intein Database and Registry, is a curated compilation of published and …

Inteins are protein segments capable of joining adjacent residues via a peptide bond. In this
process known as protein splicing, the intein itself is not present in the final sequence, thus …

Inteins are nature's escape artists; they facilitate their excision from flanking polypeptides
(exteins) concomitant with extein ligation to produce a mature host protein. Splicing requires …

Inteins are protein segments that are capable of enabling the ligation of flanking extein into a
new protein, a process known as protein splicing. Since its discovery, inteins have become …

One of the first hurdles to be negotiated in the postgenomic era involves the description of
the entire protein content of the cell, the proteome. Such efforts are presently complicated by …

Inteins are internal protein elements that self-excise from their host protein and catalyze
ligation of the flanking sequences (exteins) with a peptide bond. They are found in …