Mass spectrometry-based structural virology

TP Wörner, TM Shamorkina, J Snijder… - Analytical …, 2020 - ACS Publications
Here we review recent developments in mass spectrometry that have enabled its growing
contribution to the field of structural virology. Evidently, there is a strong renewed general …

Alphavirus RNA replication in vertebrate cells

T Ahola, G McInerney, A Merits - Advances in virus research, 2021 - Elsevier
Alphaviruses are positive-strand RNA viruses, typically transmitted by mosquitoes between
vertebrate hosts. They encode four essential replication proteins, the non-structural proteins …

Molecular architecture of the Chikungunya virus replication complex

YB Tan, D Chmielewski, MCY Law, K Zhang, Y He… - Science …, 2022 - science.org
To better understand how positive-strand (+) RNA viruses assemble membrane-associated
replication complexes (RCs) to synthesize, process, and transport viral RNA in virus-infected …

Structural insights into viral RNA capping and plasma membrane targeting by Chikungunya virus nonstructural protein 1

K Zhang, YS Law, MCY Law, YB Tan, M Wirawan… - Cell host & …, 2021 - cell.com
Chikungunya virus (CHIKV) causes a debilitating arthralgic inflammatory disease in
humans. The multifunctional CHIKV protein, nsP1, facilitates virus RNA replication and …

Architecture of the chikungunya virus replication organelle

T Laurent, P Kumar, S Liese, F Zare, M Jonasson… - Elife, 2022 - elifesciences.org
Alphaviruses are mosquito-borne viruses that cause serious disease in humans and other
mammals. Along with its mosquito vector, the Alphavirus chikungunya virus (CHIKV) has …

Crystal structures of alphavirus nonstructural protein 4 (nsP4) reveal an intrinsically dynamic RNA-dependent RNA polymerase fold

YB Tan, LS Lello, X Liu, YS Law, C Kang… - Nucleic acids …, 2022 - academic.oup.com
Abstract Alphaviruses such as Ross River virus (RRV), chikungunya virus (CHIKV), Sindbis
virus (SINV), and Venezuelan equine encephalitis virus (VEEV) are mosquito-borne …

De novo modelling of HEV replication polyprotein: Five-domain breakdown and involvement of flexibility in functional regulation

S Fieulaine, T Tubiana, S Bressanelli - Virology, 2023 - Elsevier
Hepatitis E virus (HEV), a major cause of acute viral hepatitis, is a single-stranded, positive-
sense RNA virus. As such, it encodes a 1700-residue replication polyprotein pORF1 that …

The life cycle of the alphaviruses: From an antiviral perspective

AM Skidmore, SB Bradfute - Antiviral research, 2023 - Elsevier
The alphaviruses are a widely distributed group of positive-sense, single stranded, RNA
viruses. These viruses are largely arthropod-borne and can be found on all populated …

Subdomain cryo-EM structure of nodaviral replication protein A crown complex provides mechanistic insights into RNA genome replication

N Unchwaniwala, H Zhan… - Proceedings of the …, 2020 - National Acad Sciences
For positive-strand RNA [(+) RNA] viruses, the major target for antiviral therapies is genomic
RNA replication, which occurs at poorly understood membrane-bound viral RNA replication …

Molecular basis of specific viral RNA recognition and 5′-end capping by the Chikungunya virus nsP1

K Zhang, MCY Law, TM Nguyen, YB Tan, M Wirawan… - Cell Reports, 2022 - cell.com
Many viruses encode RNA-modifying enzymes to edit the 5′ end of viral RNA to mimic the
cellular mRNA for effective protein translation, genome replication, and evasion of the host …