Human pluripotent stem cell culture: considerations for maintenance, expansion, and therapeutics

KG Chen, BS Mallon, RDG McKay, PG Robey - Cell stem cell, 2014 - cell.com
Human pluripotent stem cells (hPSCs) provide powerful resources for application in
regenerative medicine and pharmaceutical development. In the past decade, various …

Induced pluripotent stem cells for cardiovascular disease modeling and precision medicine: a scientific statement from the American Heart Association

K Musunuru, F Sheikh, RM Gupta… - Circulation: Genomic …, 2018 - Am Heart Assoc
Induced pluripotent stem cells (iPSCs) offer an unprece-dented opportunity to study human
physiology and disease at the cellular level. They also have the potential to be leveraged in …

Modelling kidney disease with CRISPR-mutant kidney organoids derived from human pluripotent epiblast spheroids

BS Freedman, CR Brooks, AQ Lam, H Fu… - Nature …, 2015 - nature.com
Abstract Human-pluripotent-stem-cell-derived kidney cells (hPSC-KCs) have important
potential for disease modelling and regeneration. Whether the hPSC-KCs can reconstitute …

Differentiation of cardiomyocytes and generation of human engineered heart tissue

K Breckwoldt, D Letuffe-Brenière, I Mannhardt… - Nature protocols, 2017 - nature.com
Since the advent of the generation of human induced pluripotent stem cells (hiPSCs),
numerous protocols have been developed to differentiate hiPSCs into cardiomyocytes and …

[HTML][HTML] Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors

K Takahashi, S Yamanaka - cell, 2006 - cell.com
Differentiated cells can be reprogrammed to an embryonic-like state by transfer of nuclear
contents into oocytes or by fusion with embryonic stem (ES) cells. Little is known about …

A fully defined and scalable 3D culture system for human pluripotent stem cell expansion and differentiation

Y Lei, DV Schaffer - … of the National Academy of Sciences, 2013 - National Acad Sciences
Human pluripotent stem cells (hPSCs), including human embryonic stem cells and induced
pluripotent stem cells, are promising for numerous biomedical applications, such as cell …

[HTML][HTML] Progress and challenges in large-scale expansion of human pluripotent stem cells

C Kropp, D Massai, R Zweigerdt - Process Biochemistry, 2017 - Elsevier
The constant supply of high cell numbers generated by defined, robust, and economically
viable culture processes is indispensable for the envisioned application of human …

Process engineering of human pluripotent stem cells for clinical application

M Serra, C Brito, C Correia, PM Alves - Trends in biotechnology, 2012 - cell.com
Human pluripotent stem cells (hPSCs), including embryonic and induced pluripotent stem
cells, constitute an extremely attractive tool for cell therapy. However, flexible platforms for …

3D bioprinting for high-throughput screening: Drug screening, disease modeling, and precision medicine applications

A Mazzocchi, S Soker, A Skardal - Applied Physics Reviews, 2019 - pubs.aip.org
High-throughput technologies have become essential in many fields of pharmaceutical and
biological development and production. Such technologies were initially developed with …

Controlling expansion and cardiomyogenic differentiation of human pluripotent stem cells in scalable suspension culture

H Kempf, R Olmer, C Kropp, M Rückert, M Jara-Avaca… - Stem cell reports, 2014 - cell.com
To harness the potential of human pluripotent stem cells (hPSCs), an abundant supply of
their progenies is required. Here, hPSC expansion as matrix-independent aggregates in …