Human pluripotent stem cell culture: considerations for maintenance, expansion, and therapeutics
Human pluripotent stem cells (hPSCs) provide powerful resources for application in
regenerative medicine and pharmaceutical development. In the past decade, various …
regenerative medicine and pharmaceutical development. In the past decade, various …
Induced pluripotent stem cells for cardiovascular disease modeling and precision medicine: a scientific statement from the American Heart Association
K Musunuru, F Sheikh, RM Gupta… - Circulation: Genomic …, 2018 - Am Heart Assoc
Induced pluripotent stem cells (iPSCs) offer an unprece-dented opportunity to study human
physiology and disease at the cellular level. They also have the potential to be leveraged in …
physiology and disease at the cellular level. They also have the potential to be leveraged in …
Modelling kidney disease with CRISPR-mutant kidney organoids derived from human pluripotent epiblast spheroids
BS Freedman, CR Brooks, AQ Lam, H Fu… - Nature …, 2015 - nature.com
Abstract Human-pluripotent-stem-cell-derived kidney cells (hPSC-KCs) have important
potential for disease modelling and regeneration. Whether the hPSC-KCs can reconstitute …
potential for disease modelling and regeneration. Whether the hPSC-KCs can reconstitute …
Differentiation of cardiomyocytes and generation of human engineered heart tissue
K Breckwoldt, D Letuffe-Brenière, I Mannhardt… - Nature protocols, 2017 - nature.com
Since the advent of the generation of human induced pluripotent stem cells (hiPSCs),
numerous protocols have been developed to differentiate hiPSCs into cardiomyocytes and …
numerous protocols have been developed to differentiate hiPSCs into cardiomyocytes and …
[HTML][HTML] Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors
K Takahashi, S Yamanaka - cell, 2006 - cell.com
Differentiated cells can be reprogrammed to an embryonic-like state by transfer of nuclear
contents into oocytes or by fusion with embryonic stem (ES) cells. Little is known about …
contents into oocytes or by fusion with embryonic stem (ES) cells. Little is known about …
A fully defined and scalable 3D culture system for human pluripotent stem cell expansion and differentiation
Y Lei, DV Schaffer - … of the National Academy of Sciences, 2013 - National Acad Sciences
Human pluripotent stem cells (hPSCs), including human embryonic stem cells and induced
pluripotent stem cells, are promising for numerous biomedical applications, such as cell …
pluripotent stem cells, are promising for numerous biomedical applications, such as cell …
[HTML][HTML] Progress and challenges in large-scale expansion of human pluripotent stem cells
C Kropp, D Massai, R Zweigerdt - Process Biochemistry, 2017 - Elsevier
The constant supply of high cell numbers generated by defined, robust, and economically
viable culture processes is indispensable for the envisioned application of human …
viable culture processes is indispensable for the envisioned application of human …
Process engineering of human pluripotent stem cells for clinical application
Human pluripotent stem cells (hPSCs), including embryonic and induced pluripotent stem
cells, constitute an extremely attractive tool for cell therapy. However, flexible platforms for …
cells, constitute an extremely attractive tool for cell therapy. However, flexible platforms for …
3D bioprinting for high-throughput screening: Drug screening, disease modeling, and precision medicine applications
A Mazzocchi, S Soker, A Skardal - Applied Physics Reviews, 2019 - pubs.aip.org
High-throughput technologies have become essential in many fields of pharmaceutical and
biological development and production. Such technologies were initially developed with …
biological development and production. Such technologies were initially developed with …
Controlling expansion and cardiomyogenic differentiation of human pluripotent stem cells in scalable suspension culture
H Kempf, R Olmer, C Kropp, M Rückert, M Jara-Avaca… - Stem cell reports, 2014 - cell.com
To harness the potential of human pluripotent stem cells (hPSCs), an abundant supply of
their progenies is required. Here, hPSC expansion as matrix-independent aggregates in …
their progenies is required. Here, hPSC expansion as matrix-independent aggregates in …