Quantitative phase imaging based on holography: trends and new perspectives

Z Huang, L Cao - Light: Science & Applications, 2024 - nature.com
Abstract In 1948, Dennis Gabor proposed the concept of holography, providing a pioneering
solution to a quantitative description of the optical wavefront. After 75 years of development …

PhaseStain: the digital staining of label-free quantitative phase microscopy images using deep learning

Y Rivenson, T Liu, Z Wei, Y Zhang, K de Haan… - Light: Science & …, 2019 - nature.com
Using a deep neural network, we demonstrate a digital staining technique, which we term
PhaseStain, to transform the quantitative phase images (QPI) of label-free tissue sections …

All‐optical phase recovery: diffractive computing for quantitative phase imaging

D Mengu, A Ozcan - Advanced Optical Materials, 2022 - Wiley Online Library
Quantitative phase imaging (QPI) is a label‐free computational imaging technique that
provides optical path length information of specimens. In modern implementations, the …

Label-free quantitative in vitro live cell imaging with digital holographic microscopy

B Kemper, A Bauwens, D Bettenworth, M Götte… - Label-Free Monitoring of …, 2019 - Springer
Label-free quantitative in vitro imaging of living cell cultures with light microscopy is an
important tool for various research fields in the life sciences. Digital holographic microscopy …

White-light diffraction tomography of unlabelled live cells

T Kim, R Zhou, M Mir, SD Babacan, PS Carney… - Nature …, 2014 - nature.com
We present a technique called white-light diffraction tomography (WDT) for imaging
microscopic transparent objects such as live unlabelled cells. The approach extends …

Quantitative 3D refractive index tomography of opaque samples in epi-mode

P Ledwig, FE Robles - Optica, 2020 - opg.optica.org
Three-dimensional (3D) refractive index (RI) tomography has recently become an exciting
new tool for biological studies. However, its limitation to (1) thin samples resulting from a …

Holographic image reconstruction with phase recovery and autofocusing using recurrent neural networks

L Huang, T Liu, X Yang, Y Luo, Y Rivenson… - ACS …, 2021 - ACS Publications
Digital holography is one of the most widely used label-free microscopy techniques in
biomedical imaging. Recovery of the missing phase information on a hologram is an …

Epi-illumination gradient light interference microscopy for imaging opaque structures

ME Kandel, C Hu, G Naseri Kouzehgarani… - Nature …, 2019 - nature.com
Multiple scattering and absorption limit the depth at which biological tissues can be imaged
with light. In thick unlabeled specimens, multiple scattering randomizes the phase of the field …

Spectroscopic phase microscopy for quantifying hemoglobin concentrations in intact red blood cells

YK Park, T Yamauchi, W Choi, R Dasari, MS Feld - Optics letters, 2009 - opg.optica.org
We report a practical method for label-free quantification of specific molecules using
spectroscopic imaging of sample-induced phase shifts. Diffraction phase microscopy …

Real-time quantitative phase imaging with a spatial phase-shifting algorithm

SK Debnath, YK Park - Optics letters, 2011 - opg.optica.org
This Letter reports on the use of a spatial phase-shifting algorithm in a fast, straightforward
method of real-time quantitative phase imaging. The computation time for phase extraction is …